Calcium Sensor for Photoacoustic Imaging

Roberts S, Seeger M, Jiang Y, Mishra A, Sigmund F, Stelzl A, Lauri A, Symvoulidis P, Rolbieski H, Preller M, Dean-Ben XL, Razansky D, Orschmann T, Desbordes SC, Vetschera P, Bach T, Ntziachristos V, Westmeyer GG (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Journal of the American Chemical Society American Chemical Society

Book Volume: 140

Pages Range: 2718-2721

Journal Issue: 8

DOI: 10.1021/jacs.7b03064

Abstract

We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca2+ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca2+ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging.

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) CSSB Centre for Structural Systems Biology DE Germany (DE)

How to cite

APA:

Roberts, S., Seeger, M., Jiang, Y., Mishra, A., Sigmund, F., Stelzl, A.,... Westmeyer, G.G. (2018). Calcium Sensor for Photoacoustic Imaging. Journal of the American Chemical Society, 140(8), 2718-2721. https://doi.org/10.1021/jacs.7b03064

MLA:

Roberts, Sheryl, et al. "Calcium Sensor for Photoacoustic Imaging." Journal of the American Chemical Society 140.8 (2018): 2718-2721.

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