X-ray phase tomography with near-field speckles for three-dimensional virtual histology
Zdora MC, Thibault P, Kuo W, Fernandez V, Deyhle H, Vila-Comamala J, Olbinado MP, Rack A, Lackie PM, Katsamenis OL, Lawson MJ, Kurtcuoglu V, Rau C, Pfeiffer F, Zanette I (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 7
Pages Range: 1221-1227
Journal Issue: 9
Abstract
High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2 mg/cm3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.
Involved external institutions
University of Southampton
United Kingdom (GB)
University of Zurich / Universität Zürich (UZH)
Switzerland (CH)
Natural History Museum London
United Kingdom (GB)
Diamond Light Source
United Kingdom (GB)
Paul Scherrer Institute (PSI)
Switzerland (CH)
European Synchrotron Radiation Facility (ESRF)
France (FR)
Technische Universität München (TUM)
Germany (DE)
Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich
Switzerland (CH)
United Kingdom (GB)
University of Zurich / Universität Zürich (UZH)
Switzerland (CH)
Natural History Museum London
United Kingdom (GB)
Diamond Light Source
United Kingdom (GB)
Paul Scherrer Institute (PSI)
Switzerland (CH)
European Synchrotron Radiation Facility (ESRF)
France (FR)
Technische Universität München (TUM)
Germany (DE)
Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich
Switzerland (CH)
How to cite
APA:
Zdora, M.-C., Thibault, P., Kuo, W., Fernandez, V., Deyhle, H., Vila-Comamala, J.,... Zanette, I. (2020). X-ray phase tomography with near-field speckles for three-dimensional virtual histology. Optica, 7(9), 1221-1227. https://doi.org/10.1364/OPTICA.399421
MLA:
Zdora, Marie-Christine, et al. "X-ray phase tomography with near-field speckles for three-dimensional virtual histology." Optica 7.9 (2020): 1221-1227.
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