Kimm MA, Tzoumas S, Glasl S, Omar M, Symvoulidis P, Olefir I, Rummeny EJ, Meier R, Ntziachristos V (2020)
Publication Type: Journal article
Publication year: 2020
Book Volume: 10
Article Number: 4903
Journal Issue: 1
DOI: 10.1038/s41598-020-61191-z
Most imaging studies of immunotherapy have focused on tracking labeled T cell biodistribution in vivo for understanding trafficking and homing parameters and predicting therapeutic efficacy by the presence of transferred T cells at or in the tumour mass. Conversely, we investigate here a novel concept for longitudinally elucidating anatomical and pathophysiological changes of solid tumours after adoptive T cell transfer in a preclinical set up, using previously unexplored in-tandem macroscopic and mesoscopic optoacoustic (photoacoustic) imaging. We show non-invasive in vivo observations of vessel collapse during tumour rejection across entire tumours and observe for the first time longitudinal tumour rejection in a label-free manner based on optical absorption changes in the tumour mass due to cellular decline. We complement these observations with high resolution episcopic fluorescence imaging of T cell biodistribution using optimized T cell labeling based on two near-infrared dyes targeting the cell membrane and the cytoplasm. We discuss how optoacoustic macroscopy and mesoscopy offer unique contrast and immunotherapy insights, allowing label-free and longitudinal observations of tumour therapy. The results demonstrate optoacoustic imaging as an invaluable tool in understanding and optimizing T cell therapy.
APA:
Kimm, M.A., Tzoumas, S., Glasl, S., Omar, M., Symvoulidis, P., Olefir, I.,... Ntziachristos, V. (2020). Longitudinal imaging of T cell-based immunotherapy with multi-spectral, multi-scale optoacoustic tomography. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-61191-z
MLA:
Kimm, Melanie A., et al. "Longitudinal imaging of T cell-based immunotherapy with multi-spectral, multi-scale optoacoustic tomography." Scientific Reports 10.1 (2020).
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