Pushing the boundaries of optoacoustic microscopy by total impulse response characterization
Seeger M, Soliman D, Aguirre J, Diot G, Wierzbowski J, Ntziachristos V (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 11
Article Number: 2910
Journal Issue: 1
DOI: 10.1038/s41467-020-16565-2
Abstract
Optical microscopy improves in resolution and signal-to-noise ratio by correcting for the system’s point spread function; a measure of how a point source is resolved, typically determined by imaging nanospheres. Optical-resolution optoacoustic (photoacoustic) microscopy could be similarly corrected, especially to account for the spatially-dependent signal distortions induced by the acoustic detection and the time-resolved and bi-polar nature of optoacoustic signals. Correction algorithms must therefore include the spatial dependence of signals’ origins and profiles in time, i.e. the four-dimensional total impulse response (TIR). However, such corrections have been so far impeded by a lack of efficient TIR-characterization methods. We introduce high-quality TIR determination based on spatially-distributed optoacoustic point sources (SOAPs), produced by scanning an optical focus on an axially-translatable 250 nm gold layer. Using a spatially-dependent TIR-correction improves the signal-to-noise ratio by >10 dB and the axial resolution by ~30%. This accomplishment displays a new performance paradigm for optoacoustic microscopy.
Involved external institutions
Germany (DE)How to cite
APA:
Seeger, M., Soliman, D., Aguirre, J., Diot, G., Wierzbowski, J., & Ntziachristos, V. (2020). Pushing the boundaries of optoacoustic microscopy by total impulse response characterization. Nature Communications, 11(1). https://doi.org/10.1038/s41467-020-16565-2
MLA:
Seeger, Markus, et al. "Pushing the boundaries of optoacoustic microscopy by total impulse response characterization." Nature Communications 11.1 (2020).
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