scPower accelerates and optimizes the design of multi-sample single cell transcriptomic studies
Schmid KT, Hoellbacher B, Cruceanu C, Boettcher A, Lickert H, Binder EB, Theis FJ, Heinig M (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 12
Article Number: 6625
Journal Issue: 1
DOI: 10.1038/s41467-021-26779-7
Abstract
Single cell RNA-seq has revolutionized transcriptomics by providing cell type resolution for differential gene expression and expression quantitative trait loci (eQTL) analyses. However, efficient power analysis methods for single cell data and inter-individual comparisons are lacking. Here, we present scPower; a statistical framework for the design and power analysis of multi-sample single cell transcriptomic experiments. We modelled the relationship between sample size, the number of cells per individual, sequencing depth, and the power of detecting differentially expressed genes within cell types. We systematically evaluated these optimal parameter combinations for several single cell profiling platforms, and generated broad recommendations. In general, shallow sequencing of high numbers of cells leads to higher overall power than deep sequencing of fewer cells. The model, including priors, is implemented as an R package and is accessible as a web tool. scPower is a highly customizable tool that experimentalists can use to quickly compare a multitude of experimental designs and optimize for a limited budget.
Involved external institutions
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Max-Planck-Institut für Psychiatrie (Deutsche Forschungsanstalt für Psychiatrie)
Germany (DE)
Germany (DE)
Max-Planck-Institut für Psychiatrie (Deutsche Forschungsanstalt für Psychiatrie)
Germany (DE)
How to cite
APA:
Schmid, K.T., Hoellbacher, B., Cruceanu, C., Boettcher, A., Lickert, H., Binder, E.B.,... Heinig, M. (2021). scPower accelerates and optimizes the design of multi-sample single cell transcriptomic studies. Nature Communications, 12(1). https://doi.org/10.1038/s41467-021-26779-7
MLA:
Schmid, Katharina T., et al. "scPower accelerates and optimizes the design of multi-sample single cell transcriptomic studies." Nature Communications 12.1 (2021).
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