Wide-Field Mid-Infrared Hyperspectral Imaging by Snapshot Phase Contrast Measurement of Optothermal Excitation
Yuan T, Pleitez MA, Gasparin F, Ntziachristos V (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 93
Pages Range: 15323-15330
Journal Issue: 46
DOI: 10.1021/acs.analchem.1c02805
Abstract
Vibrational microscopy methods based on Raman scattering or infrared absorption provide a label-free approach for chemical-contrast imaging, but employ point-by-point scanning and impose a compromise between the imaging speed and field-of-view (FOV). Optothermal microscopy has been proposed as a promising imaging modality to avoid this compromise, although at restrictively small FOVs capable of imaging only few cells. Here, we present wide-field optothermal mid-infrared microscopy (WOMiM) for wide-field chemical-contrast imaging based on snapshot pump-probe detection of optothermal signal, using a custom-made condenser-free phase contrast microscopy to capture the phase change of samples after mid-infrared irradiation. We achieved chemical contrast for FOVs up to 180 μm in diameter, yielding 10-fold larger imaging areas than the state-of-the-art, at imaging speeds of 1 ms/frame. The maximum possible imaging speed of WOMiM was determined by the relaxation time of optothermal heat, measured to be 32.8 μs in water, corresponding to a frame rate of ∼30 kHz. This proof-of-concept demonstrates that vibrational imaging can be achieved at an unprecedented imaging speed and large FOV with the potential to significantly facilitate label-free imaging of cellular dynamics.
Involved external institutions
Germany (DE)How to cite
APA:
Yuan, T., Pleitez, M.A., Gasparin, F., & Ntziachristos, V. (2021). Wide-Field Mid-Infrared Hyperspectral Imaging by Snapshot Phase Contrast Measurement of Optothermal Excitation. Analytical Chemistry, 93(46), 15323-15330. https://doi.org/10.1021/acs.analchem.1c02805
MLA:
Yuan, Tao, et al. "Wide-Field Mid-Infrared Hyperspectral Imaging by Snapshot Phase Contrast Measurement of Optothermal Excitation." Analytical Chemistry 93.46 (2021): 15323-15330.
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