Acute radiation syndrome-related gene expression in irradiated peripheral blood cell populations
Ostheim P, Don Mallawaratchy A, Mueller T, Schuele S, Hermann C, Popp T, Eder S, Combs SE, Port M, Abend M (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 97
Pages Range: 474-484
Journal Issue: 4
DOI: 10.1080/09553002.2021.1876953
Abstract
Purpose: In a nuclear or radiological event, an early diagnostic tool is needed to distinguish the worried well from those individuals who may later develop life-threatenFing hematologic acute radiation syndrome. We examined the contribution of the peripheral blood's cell populations on radiation-induced gene expression (GE) changes. Materials and methods: EDTA-whole-blood from six healthy donors was X-irradiated with 0 and 4Gy and T-lymphocytes, B-lymphocytes, NK-cells and granulocytes were separated using immunomagnetic methods. GE were examined in cell populations and whole blood. Results: The cell populations contributed to the total RNA amount with a ratio of 11.6 for T-lymphocytes, 1.2 for B-cells, 1.2 for NK-cells, 1.0 for granulocytes. To estimate the contribution of GE per cell population, the baseline (0Gy) and the radiation-induced fold-change in GE relative to unexposed was considered for each gene. The T-lymphocytes (74.8%/80.5%) contributed predominantly to the radiation-induced up-regulation observed for FDXR/DDB2 and the B-lymphocytes (97.1%/83.8%) for down-regulated POU2AF1/WNT3 with a similar effect on whole blood gene expression measurements reflecting a corresponding order of magnitude. Conclusions: T- and B-lymphocytes contributed predominantly to the radiation-induced up-regulation of FDXR/DDB2 and down-regulation of POU2AF1/WNT3. This study underlines the use of FDXR/DDB2 for biodosimetry purposes and POU2AF1/WNT3 for effect prediction of acute health effects.
Involved external institutions
Germany (DE)How to cite
APA:
Ostheim, P., Don Mallawaratchy, A., Mueller, T., Schuele, S., Hermann, C., Popp, T.,... Abend, M. (2021). Acute radiation syndrome-related gene expression in irradiated peripheral blood cell populations. International Journal of Radiation Biology, 97(4), 474-484. https://doi.org/10.1080/09553002.2021.1876953
MLA:
Ostheim, Patrick, et al. "Acute radiation syndrome-related gene expression in irradiated peripheral blood cell populations." International Journal of Radiation Biology 97.4 (2021): 474-484.
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