Generation of heterozygous (MRli003-A-3) and homozygous (MRli003-A-4) TRPM4 knockout human iPSC lines

Zhang F, Meier ABB, Lipp P, Laugwitz KL, Dorn T, Moretti A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Stem Cell Research Elsevier

Book Volume: 60

Article Number: 102731

DOI: 10.1016/j.scr.2022.102731

Abstract

TRPM4 is a Ca2+-activated channel mediating the transport of monovalent cations across the cell membrane. Mutations in the TRPM4 gene have been associated with cardiac arrhythmias in humans. Using CRISPR/Cas9 gene editing technology, we established two TRPM4 knockout human iPSC lines – one heterozygous (MRli003-A-3) and one homozygous (MRli003-A-4) – by inserting a frameshift mutation in exon 2 of the TRPM4 gene. Both lines maintained pluripotency, a normal karyotype, parental cell morphology, and the ability to differentiate into the three germ layers.

Involved external institutions

Universität des Saarlandes (UdS) DE Germany (DE) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Zhang, F., Meier, A.B.B., Lipp, P., Laugwitz, K.-L., Dorn, T., & Moretti, A. (2022). Generation of heterozygous (MRli003-A-3) and homozygous (MRli003-A-4) TRPM4 knockout human iPSC lines. Stem Cell Research, 60. https://doi.org/10.1016/j.scr.2022.102731

MLA:

Zhang, Fangfang, et al. "Generation of heterozygous (MRli003-A-3) and homozygous (MRli003-A-4) TRPM4 knockout human iPSC lines." Stem Cell Research 60 (2022).

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