Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus

Zhang F, Meier AB, Sinnecker D, Engelhardt S, Lipp P, Laugwitz KL, Dorn T, Moretti A (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Stem Cell Research Elsevier

Book Volume: 61

Article Number: 102785

DOI: 10.1016/j.scr.2022.102785

Abstract

Assessment of the electrophysiological properties of cardiomyocytes is necessary for phenotyping cardiac disorders and for drug screening. Optical action potential imaging using a genetically encoded voltage-sensing fluorescent protein (VSFP) allows for high-throughput functional characterization of cardiomyocytes, which offers an advantage over the traditional patch-clamp technique. Here, we knocked VSFP into the AAVS1 safe harbor locus of human iPSCs, generating two stable voltage indicator lines - one heterozygous (MRIi003-A-5) and the other homozygous (MRI003-A-6). Both lines can be used for optical membrane potential recordings and provide a powerful platform for a wide range of applications in cardiovascular biomedicine.

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) Universität des Saarlandes (UdS) DE Germany (DE) Deutsches Zentrum für Herz-Kreislauf-Forschung (DZHK e.V.) DE Germany (DE)

How to cite

APA:

Zhang, F., Meier, A.B., Sinnecker, D., Engelhardt, S., Lipp, P., Laugwitz, K.-L.,... Moretti, A. (2022). Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus. Stem Cell Research, 61. https://doi.org/10.1016/j.scr.2022.102785

MLA:

Zhang, Fangfang, et al. "Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus." Stem Cell Research 61 (2022).

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