In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells

Kraus A, Lüdke A, Teschemacher B, Skoczynski K, Buchholz B (2023)

Publication Type: Journal article

Publication year: 2023

Journal

STAR Protocols Cell Press

Book Volume: 4

Article Number: 101874

Journal Issue: 1

DOI: 10.1016/j.xpro.2022.101874

Abstract

Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to induce tubule formation. We detail steps for the establishment of the in vitro cyst assay, followed by puncture of the cysts in the collagen matrix. We further describe live imaging and steps to analyze the tubule growth. For complete details on the use and execution of this protocol, please refer to Scholz et al. (2022).¹

Authors with CRIS profile

Andre Kraus Department of Medicine 4 – Nephrology and Hypertension Björn Buchholz Medizinische Fakultät

How to cite

APA:

Kraus, A., Lüdke, A., Teschemacher, B., Skoczynski, K., & Buchholz, B. (2023). In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells. STAR Protocols, 4(1). https://doi.org/10.1016/j.xpro.2022.101874

MLA:

Kraus, Andre, et al. "In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells." STAR Protocols 4.1 (2023).

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