Lavage-induced surfactant depletion in pigs as a model of the acute respiratory distress syndrome (ARDS)
Russ M, Kronfeldt S, Boemke W, Busch T, Francis RCE, Pickerodt PA (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 2016
Article Number: e53610
Journal Issue: 115
DOI: 10.3791/53610
Abstract
Various animal models of lung injury exist to study the complex pathomechanisms of human acute respiratory distress syndrome (ARDS) and evaluate future therapies. Severe lung injury with a reproducible deterioration of pulmonary gas exchange and hemodynamics can be induced in anesthetized pigs using repeated lung lavages with warmed 0.9% saline (50 ml/kg body weight). Including standard respiratory and hemodynamic monitoring with clinically applied devices in this model allows the evaluation of novel therapeutic strategies (drugs, modern ventilators, extracorporeal membrane oxygenators, ECMO), and bridges the gap between bench and bedside. Furthermore, induction of lung injury with lung lavages does not require the injection of pathogens/endotoxins that impact on measurements of pro- and anti-inflammatory cytokines. A disadvantage of the model is the high recruitability of atelectatic lung tissue. Standardization of the model helps to avoid pitfalls, to ensure comparability between experiments, and to reduce the number of animals needed.
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Germany (DE)How to cite
APA:
Russ, M., Kronfeldt, S., Boemke, W., Busch, T., Francis, R.C.E., & Pickerodt, P.A. (2016). Lavage-induced surfactant depletion in pigs as a model of the acute respiratory distress syndrome (ARDS). Journal of Visualized Experiments, 2016(115). https://doi.org/10.3791/53610
MLA:
Russ, Martin, et al. "Lavage-induced surfactant depletion in pigs as a model of the acute respiratory distress syndrome (ARDS)." Journal of Visualized Experiments 2016.115 (2016).
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