Mature osteoblasts dedifferentiate in response to traumatic bone injury in the zebrafish fin and skull
Geurtzen K, Knopf F, Wehner D, Huitema LFA, Schulte-Merker S, Weidinger G (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Book Volume: 141
Pages Range: 2225-2234
Journal Issue: 11
DOI: 10.1242/dev.105817
Abstract
Zebrafish have an unlimited capacity to regenerate bone after fin amputation. In this process, mature osteoblasts dedifferentiate to osteogenic precursor cells and thus represent an important source of newly forming bone. By contrast, differentiated osteoblasts do not appear to contribute to repair of bone injuries in mammals; rather, osteoblasts form anew from mesenchymal stem cells. This raises the question whether osteoblast dedifferentiation is specific to appendage regeneration, a special feature of the lepidotrichia bone of the fish fin, or a process found more generally in fish bone. Here, we show that dedifferentiation of mature osteoblasts is not restricted to fin regeneration afteramputation, but also occurs during repair of zebrafish fin fractures and skull injuries. In both models, mature osteoblasts surrounding the injury downregulate the expression of differentiation markers, upregulate markers of the pre-osteoblast state and become proliferative. Making use of photoconvertible Kaede protein as well as Cre-driven genetic fate mapping, we show that osteoblasts migrate to the site of injury to replace damaged tissue. Our findings suggest a fundamental role for osteoblast dedifferentiation in reparative bone formation in fish and indicate that adult fish osteoblasts display elevated cellular plasticity compared with mammalian bone-forming cells. © 2014. Published by The Company of Biologists Ltd.
Involved external institutions
Technische Universität Dresden
Germany (DE)
Universität Ulm
Germany (DE)
Royal Netherlands Academy of Arts and Sciences / Koninklijke Nederlandse Akademie van Wetenschappen (KNAW)
Netherlands (NL)
Germany (DE)
Universität Ulm
Germany (DE)
Royal Netherlands Academy of Arts and Sciences / Koninklijke Nederlandse Akademie van Wetenschappen (KNAW)
Netherlands (NL)
How to cite
APA:
Geurtzen, K., Knopf, F., Wehner, D., Huitema, L.F.A., Schulte-Merker, S., & Weidinger, G. (2014). Mature osteoblasts dedifferentiate in response to traumatic bone injury in the zebrafish fin and skull. Development, 141(11), 2225-2234. https://doi.org/10.1242/dev.105817
MLA:
Geurtzen, Karina, et al. "Mature osteoblasts dedifferentiate in response to traumatic bone injury in the zebrafish fin and skull." Development 141.11 (2014): 2225-2234.
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