Identification of Murine Basophils by Flow Cytometry and Histology.

Schwartz C, Vöhringer D (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Methods in Molecular Biology Humana Press

Book Volume: 2163

Pages Range: 367-375

DOI: 10.1007/978-1-0716-0696-4_29

Abstract

Here, we describe how murine basophils can be detected in vivo by flow cytometry and immunofluorescence staining. Basophils constitute a homogeneous population of CD4-CD19-CD49b+IgE+ cells in flow cytometric analysis. When IgE levels are low, one can also use anti-FcεRI or anti-CD200R3 antibodies instead of anti-IgE. For immunofluorescence staining, we use an anti-Mcpt8 antibody since Mcpt8 is a specific marker for murine basophils. We describe how to prepare the tissue to cut cryo-sections and how to perform the staining using a tyramide-based amplification kit.

Authors with CRIS profile

Christian Schwartz Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene David Vöhringer Professur für Infektionsabwehr und Toleranz

How to cite

APA:

Schwartz, C., & Vöhringer, D. (2020). Identification of Murine Basophils by Flow Cytometry and Histology. Methods in Molecular Biology, 2163, 367-375. https://doi.org/10.1007/978-1-0716-0696-4_29

MLA:

Schwartz, Christian, and David Vöhringer. "Identification of Murine Basophils by Flow Cytometry and Histology." Methods in Molecular Biology 2163 (2020): 367-375.

BibTeX: Download