Artificial Organelles with Orthogonal-Responsive Membranes for Protocell Systems: Probing the Intrinsic and Sequential Docking and Diffusion of Cargo into Two Coexisting Avidin–Polymersomes

Wang X, Moreno S, Boye S, Wang P, Liu X, Lederer A, Voit B, Appelhans D (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Advanced Science Wiley Open Access

Book Volume: 8

Article Number: 2004263

Journal Issue: 11

DOI: 10.1002/advs.202004263

Abstract

The challenge of effective integration and use of artificial organelles with orthogonal-responsive membranes and their communication in eukaryotic protocells is to understand the intrinsic membrane characteristics. Here, a novel photo-crosslinked and pH-responsive polymersome (Psome B) with 2-(N,N′-diisopropylamino)ethyl units in the membrane and its respective Avidin-Psome B hybrids, are reported as good candidates for artificial organelles. Biotinylated (macro)molecules are able to dock and diffuse into Avidin-Psome B to carry out biological activity in a pH- and size-dependent manner. Combined with another polymersome (Psome A) with 2-(N,N′-diethylamino)ethyl units in the membrane, two different pH-responsive polymersomes for mimicking different organelles in one protocell system are reported. The different intrinsic docking and diffusion processes of cargo (macro)molecules through the membranes of coexisting Psome A and B are pH-dependent as confirmed using pH titration–dynamic light scattering (DLS). Psome A and B show separated “open”, “closing/opening”, and “closed” states at various pH ranges with different membrane permeability. The results pave the way for the construction of multicompartmentalized protocells with controlled communications between different artificial organelles.

Involved external institutions

Leibniz-Institut für Polymerforschung Dresden e. V. DE Germany (DE) Sichuan University (SCU) / 四川大学 CN China (CN)

How to cite

APA:

Wang, X., Moreno, S., Boye, S., Wang, P., Liu, X., Lederer, A.,... Appelhans, D. (2021). Artificial Organelles with Orthogonal-Responsive Membranes for Protocell Systems: Probing the Intrinsic and Sequential Docking and Diffusion of Cargo into Two Coexisting Avidin–Polymersomes. Advanced Science, 8(11). https://doi.org/10.1002/advs.202004263

MLA:

Wang, Xueyi, et al. "Artificial Organelles with Orthogonal-Responsive Membranes for Protocell Systems: Probing the Intrinsic and Sequential Docking and Diffusion of Cargo into Two Coexisting Avidin–Polymersomes." Advanced Science 8.11 (2021).

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