DMBT1 amount in amniotic fluid depends on gestational age

Blickwedel J, Bagci S, Alsat EA, Strizek B, Renner M, Mueller A, Mueller H (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Journal of Maternal-Fetal & Neonatal Medicine Informa Healthcare

DOI: 10.1080/14767058.2021.1937103

Abstract

Objective Amniotic fluid is a mixture containing many different proteins as immunomodulatory peptides and growth factors. The glycoprotein Deleted in Malignant Brain Tumors 1 (DMBT1) is participated in innate immunity, angiogenesis and epithelial differentiation. We analyzed the DMBT1 concentration in amniotic fluid during gestation. Methods DMBT1 concentration was quantified by ELISA. Amniotic fluid samples were collected from preterm and term neonates. Effects of maternal or neonatal parameters were analyzed. To evaluate the source of DMBT1 we examined RNA of fetal tissue in relation to DMBT1 expression. Results The median DMBT1 concentration in amniotic fluid was 54.4 ng/ml. Amniotic fluid obtained <28 weeks of gestation revealed significantly lower DMBT1 concentrations compared to >= 28 weeks. We found a positive correlation between DMBT1 concentration and gestational age (p = .026). The fetal DMBT1 expression was pronounced in the gastrointestinal tract. Conclusions The results showed that DMBT1 concentrations in amniotic fluid correlate with the gestational age during gestation and that the fetal gastrointestinal tract is a potential source of DMBT1. BRIEF RATIONALE Amniotic fluid contains not only nutrients, but also many immunomodulatory peptides and growth factors. Deleted in Malignant Brain Tumors 1 (DMBT1) is an innate immunity protein with functions in epithelial differentiation and angiogenesis. The aim of this research was to study the DMBT1 content and the factors affecting its concentration in amniotic fluid during gestation. In summary, the results obtained in this study showed that DMBT1 is a component of amniotic fluid and that DMBT1 concentrations in amniotic fluid correlate with gestational age. In addition to this, the fetal gastrointestinal tract is a potential source of DMBT1 detected in amniotic fluid.

Additional Organisation(s)

Department of Paediatrics and Adolescent Medicine

Involved external institutions

Rheinische Friedrich-Wilhelms-Universität Bonn DE Germany (DE) Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Philipps-Universität Marburg DE Germany (DE)

How to cite

APA:

Blickwedel, J., Bagci, S., Alsat, E.A., Strizek, B., Renner, M., Mueller, A., & Mueller, H. (2021). DMBT1 amount in amniotic fluid depends on gestational age. Journal of Maternal-Fetal & Neonatal Medicine. https://doi.org/10.1080/14767058.2021.1937103

MLA:

Blickwedel, Jana, et al. "DMBT1 amount in amniotic fluid depends on gestational age." Journal of Maternal-Fetal & Neonatal Medicine (2021).

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