Inter-protein interactions govern protein loading into porous vaterite CaCO3crystals
Feoktistova NA, Balabushevich NG, Skirtach AG, Volodkin D, Vikulina AS (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 22
Pages Range: 9713-9722
Journal Issue: 17
DOI: 10.1039/d0cp00404a
Abstract
The fast development of protein therapeutics has resulted in a high demand for advanced delivery carriers that can effectively host therapeutic proteins, preserve their bioactivity and release them on demand. Accordingly, vaterite CaCO3 crystals have attracted special attention as sacrificial templates for protein encapsulation in micro- and nanoparticles (capsules and beads, respectively) under mild biofriendly conditions. This study aimed to better understand the mechanism of protein loading into crystals as a primary step for protein encapsulation. The loading of three therapeutic proteins (250 kDa catalase, 5.8 kDa insulin, and 6.5 kDa aprotinin) was investigated for crystals with different porosities. However, unexpectedly, the protein loading capacity was not consistent with the protein molecular weight. It solely depends on the inter-protein interactions in the bulk solution in the presence of crystals and that inside the crystals. The smallest protein aprotinin aggregates in the bulk (its aggregate size is about 100 nm), which prohibits its loading into the crystals. Insulin forms hexamers in the bulk, which can diffuse into the crystal pores but tend to aggregate inside the pores, suppressing protein diffusion inward. Catalase, the largest protein tested, does not form any aggregates in the bulk and diffuses freely into the crystals; however, its diffusion into small pores is sterically restricted. These findings are essential for the encapsulation of protein therapeutics by means of templating based on CaCO3 crystals and for the engineering of protein-containing microparticles having desired architectures.
Authors with CRIS profile
Involved external institutions
Lomonosov Moscow State University / Московский государственный университет имени М.В.Ломоносова
Russian Federation (RU)
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI)
Germany (DE)
Universiteit Gent (UGent) / Ghent University
Belgium (BE)
Russian Federation (RU)
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI)
Germany (DE)
Universiteit Gent (UGent) / Ghent University
Belgium (BE)
How to cite
APA:
Feoktistova, N.A., Balabushevich, N.G., Skirtach, A.G., Volodkin, D., & Vikulina, A.S. (2020). Inter-protein interactions govern protein loading into porous vaterite CaCO3crystals. Physical Chemistry Chemical Physics, 22(17), 9713-9722. https://doi.org/10.1039/d0cp00404a
MLA:
Feoktistova, Natalia A., et al. "Inter-protein interactions govern protein loading into porous vaterite CaCO3crystals." Physical Chemistry Chemical Physics 22.17 (2020): 9713-9722.
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