Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy

Matthies L, Gebrekidan M, Tegtmeyer JF, Oetter N, Rohde M, Vollkommer T, Smeets R, Wilczak W, Stelzle F, Gosau M, Braeuer AS, Knipfer C (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Biomedical Optics Express Optical Society of America

Book Volume: 12

Pages Range: 836-851

Journal Issue: 2

DOI: 10.1364/BOE.409456

Abstract

Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers and frequently preceded by non-malignant lesions. Using Shifted-Excitation Raman Difference Spectroscopy (SERDS), principal component and linear discriminant analysis in native tissue specimens, 9500 raw Raman spectra of OSCC, 4300 of non-malignant lesions and 4200 of physiological mucosa were evaluated. Non-malignant lesions were distinguished from physiological mucosa with a classification accuracy of 95.3% (95.4% sensitivity, 95.2% specificity, area under the curve (AUC) 0.99). Discriminating OSCC from non-malignant lesions showed an accuracy of 88.4% (93.7% sensitivity, 76.7% specificity, AUC 0.93). OSCC was identified against physiological mucosa with an accuracy of 89.8% (93.7% sensitivity, 81.0% specificity, AUC 0.90). These findings underline the potential of SERDS for the diagnosis of oral cavity lesions.

Authors with CRIS profile

Medhanie Gebrekidan Erlangen Graduate School in Advanced Optical Technologies Nicolai Oetter Department of Oral and Cranio-Maxillofacial Surgery Maximilian Rohde Department of Oral and Cranio-Maxillofacial Surgery Florian Stelzle Department of Oral and Cranio-Maxillofacial Surgery

Involved external institutions

Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Technische Universität Bergakademie Freiberg DE Germany (DE)

How to cite

APA:

Matthies, L., Gebrekidan, M., Tegtmeyer, J.F., Oetter, N., Rohde, M., Vollkommer, T.,... Knipfer, C. (2021). Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy. Biomedical Optics Express, 12(2), 836-851. https://doi.org/10.1364/BOE.409456

MLA:

Matthies, Levi, et al. "Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy." Biomedical Optics Express 12.2 (2021): 836-851.

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