Specific Induction of Double Negative B Cells During Protective and Pathogenic Immune Responses
Ruschil C, Gabernet G, Lepennetier G, Heumos S, Kaminski M, Hracsko Z, Irmler M, Beckers J, Ziemann U, Nahnsen S, Owens GP, Bennett JL, Hemmer B, Kowarik MC (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 11
DOI: 10.3389/fimmu.2020.606338
Abstract
Double negative (DN) (CD19(+)CD20(low)CD27(-)IgD(-)) B cells are expanded in patients with autoimmune and infectious diseases; however their role in the humoral immune response remains unclear. Using systematic flow cytometric analyses of peripheral blood B cell subsets, we observed an inflated DN B cell population in patients with variety of active inflammatory conditions: myasthenia gravis, Guillain-Barre syndrome, neuromyelitis optica spectrum disorder, meningitis/encephalitis, and rheumatic disorders. Furthermore, we were able to induce DN B cells in healthy subjects following vaccination against influenza and tick borne encephalitis virus. Transcriptome analysis revealed a gene expression profile in DN B cells that clustered with naive B cells, memory B cells, and plasmablasts. Immunoglobulin VH transcriptome sequencing and analysis of recombinant antibodies revealed clonal expansion of DN B cells that were targeted against the vaccine antigen. Our study suggests that DN B cells are expanded in multiple inflammatory neurologic diseases and represent an inducible B cell population that responds to antigenic stimulation, possibly through an extra-follicular maturation pathway.
Involved external institutions
Technische Universität München (TUM)
Germany (DE)
Eberhard Karls Universität Tübingen
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Charité - Universitätsmedizin Berlin
Germany (DE)
University of Colorado System
United States (USA) (US)
Germany (DE)
Eberhard Karls Universität Tübingen
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Charité - Universitätsmedizin Berlin
Germany (DE)
University of Colorado System
United States (USA) (US)
How to cite
APA:
Ruschil, C., Gabernet, G., Lepennetier, G., Heumos, S., Kaminski, M., Hracsko, Z.,... Kowarik, M.C. (2020). Specific Induction of Double Negative B Cells During Protective and Pathogenic Immune Responses. Frontiers in Immunology, 11. https://doi.org/10.3389/fimmu.2020.606338
MLA:
Ruschil, Christoph, et al. "Specific Induction of Double Negative B Cells During Protective and Pathogenic Immune Responses." Frontiers in Immunology 11 (2020).
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