"zebrafishing" for novel genes relevant to the glomerular filtration barrier
Hanke N, Staggs L, Schroder P, Litteral J, Fleig S, Kaufeld J, Pauli C, Haller H, Schiffer M (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 2013
Article Number: 658270
DOI: 10.1155/2013/658270
Abstract
Data for genes relevant to glomerular filtration barrier function or proteinuria is continually increasing in an era of microarrays, genome-wide association studies, and quantitative trait locus analysis. Researchers are limited by published literature searches to select the most relevant genes to investigate. High-throughput cell cultures and other in vitro systems ultimately need to demonstrate proof in an in vivo model. Generating mammalian models for the genes of interest is costly and time intensive, and yields only a small number of test subjects. These models also have many pitfalls such as possible embryonic mortality and failure to generate phenotypes or generate nonkidney specific phenotypes. Here we describe an in vivo zebrafish model as a simple vertebrate screening system to identify genes relevant to glomerular filtration barrier function. Using our technology, we are able to screen entirely novel genes in 4-6 weeks in hundreds of live test subjects at a fraction of the cost of a mammalian model. Our system produces consistent and reliable evidence for gene relevance in glomerular kidney disease; the results then provide merit for further analysis in mammalian models. © 2013 Nils Hanke et al.
Authors with CRIS profile
Involved external institutions
Medizinische Hochschule Hannover (MHH) / Hannover Medical School
Germany (DE)
Mount Desert Island Biological Laboratory
United States (USA) (US)
Germany (DE)
Mount Desert Island Biological Laboratory
United States (USA) (US)
How to cite
APA:
Hanke, N., Staggs, L., Schroder, P., Litteral, J., Fleig, S., Kaufeld, J.,... Schiffer, M. (2013). "zebrafishing" for novel genes relevant to the glomerular filtration barrier. BioMed Research International, 2013. https://doi.org/10.1155/2013/658270
MLA:
Hanke, Nils, et al. ""zebrafishing" for novel genes relevant to the glomerular filtration barrier." BioMed Research International 2013 (2013).
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