In vivo modulation of angiogenesis and immune response on a collagen matrix via extracorporeal shockwaves

Heimes D, Wiesmann N, Eckrich J, Brieger J, Mattyasovszky S, Proff P, Weber M, Deschner J, Al-Nawas B, Kämmerer PW (2020)

Publication Type: Journal article

Publication year: 2020

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 21

Pages Range: 1-26

Article Number: 7574

Journal Issue: 20

DOI: 10.3390/ijms21207574

Abstract

The eαective management of tissue integration and immunological responses to transplants decisively co-determines the success of soft and hard tissue reconstruction. The aim of this in vivo study was to evaluate the eligibility of extracorporeal shock wave therapy (ESWT) with respect to its ability to modulate angiogenesis and immune response to a collagen matrix (CM) for tissue engineering in the chorioallantoic membrane (CAM) assay, which is performed with fertilized chicken eggs. CM were placed on the CAM on embryonic development day (EDD) 7; at EDD-10, ESWT was conducted at 0.12 mJ/mm2 with 500 impulses each. One and four days later, angiogenesis represented by vascularized area, vessel density, and vessel junctions as well as HIF-1α and VEGF gene expression were evaluated. Furthermore, immune response (iNOS2, MMP-9, and MMP-13 via qPCR) was assessed and compared between ESWT- and non-ESWT-groups. At EDD-14, the vascularized area (+115% vs. +26%) and the increase in vessel junctions (+751% vs. +363%) were significantly higher in the ESWT-group. ESWT significantly increased MMP-9 gene expression at EDD-11 and significantly decreased MMP-13 gene expression at EDD-14 as compared to the controls. Using the CAM assay, an enhanced angiogenesis and neovascularization in CM after ESWT were observed. Furthermore, ESWT could reduce the inflammatory activity after a latency of four days.

Authors with CRIS profile

Manuel Weber Department of Oral and Cranio-Maxillofacial Surgery

Involved external institutions

Universitätsmedizin der Johannes Gutenberg-Universität Mainz DE Germany (DE) Universitätsklinikum Regensburg DE Germany (DE)

How to cite

APA:

Heimes, D., Wiesmann, N., Eckrich, J., Brieger, J., Mattyasovszky, S., Proff, P.,... Kämmerer, P.W. (2020). In vivo modulation of angiogenesis and immune response on a collagen matrix via extracorporeal shockwaves. International Journal of Molecular Sciences, 21(20), 1-26. https://doi.org/10.3390/ijms21207574

MLA:

Heimes, Diana, et al. "In vivo modulation of angiogenesis and immune response on a collagen matrix via extracorporeal shockwaves." International Journal of Molecular Sciences 21.20 (2020): 1-26.

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