Role of the small Rho GTPases Rac1 and Cdc42 in host cell invasion of Campylobacter jejuni

Krause-Gruszczynska M, Rohde M, Hartig R, Genth H, Schmid G, Keo T, König W, Miller WG, Konkel ME, Backert S (2007)

Publication Type: Journal article

Publication year: 2007

Journal

Cellular Microbiology Wiley

Book Volume: 9

Pages Range: 2431-2444

Journal Issue: 10

DOI: 10.1111/j.1462-5822.2007.00971.x

Abstract

Host cell invasion of the food-borne pathogen Campylobacter jejuni is one of the primary reasons of tissue damage in humans but molecular mechanisms are widely unclear. Here, we show that C. jejuni triggers membrane ruffling in the eukaryotic cell followed by invasion in a very specific manner first with its tip followed by the flagellar end. To pinpoint important signalling events involved in the C.jejuni invasion process, we examined the role of small Rho family GTPases. Using specific GTPase-modifying toxins, inhibitors and GTPase expression constructs we show that Rac1 and Cdc42, but not RhoA, are involved in C.jejuni invasion. In agreement with these observations, we found that internalization of C.jejuni is accompanied by a time-dependent activation of both Rac1 and Cdc42. Finally, we show that the activation of these GTPases involves different host cell kinases and the bacterial fibronectin-binding protein CadF. Thus, CadF is a bifunctional protein which triggers bacterial binding to host cells as well as signalling leading to GTPase activation. Collectively, our results suggest that C.jejuni invade host target cells by a unique mechanism and the activation of the Rho GTPase members Rac1 and Cdc42 plays a crucial role in this entry process. © 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltd.

Authors with CRIS profile

Steffen Backert

Involved external institutions

Washington State University (WSU) US United States (USA) (US) New York University (NYU) US United States (USA) (US) Agricultural Research Service (USDA ARS) US United States (USA) (US) Otto-von-Guericke-Universität Magdeburg DE Germany (DE) Helmholtz-Zentrum für Infektionsforschung (HZI) DE Germany (DE) Albert-Ludwigs-Universität Freiburg DE Germany (DE) Medizinische Hochschule Hannover (MHH) / Hannover Medical School DE Germany (DE)

How to cite

APA:

Krause-Gruszczynska, M., Rohde, M., Hartig, R., Genth, H., Schmid, G., Keo, T.,... Backert, S. (2007). Role of the small Rho GTPases Rac1 and Cdc42 in host cell invasion of Campylobacter jejuni. Cellular Microbiology, 9(10), 2431-2444. https://doi.org/10.1111/j.1462-5822.2007.00971.x

MLA:

Krause-Gruszczynska, Malgorzata, et al. "Role of the small Rho GTPases Rac1 and Cdc42 in host cell invasion of Campylobacter jejuni." Cellular Microbiology 9.10 (2007): 2431-2444.

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