Calcium phosphate nanoparticle-based vaccines as a platform for improvement of HIV-1 env antibody responses by intrastructural help

Damm D, Rojas-Sánchez L, Theobald H, Sokolova V, Wyatt RT, Überla K, Epple M, Temchura V (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Nanomaterials MDPI

Book Volume: 9

Article Number: 1389

Journal Issue: 10

DOI: 10.3390/nano9101389

Abstract

Incorporation of immunodominant T-helper epitopes of licensed vaccines into virus-like particles (VLP) allows to harness T-helper cells induced by the licensed vaccines to provide intrastructural help (ISH) for B-cell responses against the surface proteins of the VLPs. To explore whether ISH could also improve antibody responses to calcium phosphate (CaP) nanoparticle vaccines we loaded the nanoparticle core with a universal T-helper epitope of Tetanus toxoid (p30) and functionalized the surface of CaP nanoparticles with stabilized trimers of the HIV-1 envelope (Env) resulting in Env-CaP-p30 nanoparticles. In contrast to soluble Env trimers, Env containing CaP nanoparticles induced activation of naïve Env-specific B-cells in vitro. Mice previously vaccinated against Tetanus raised stronger humoral immune responses against Env after immunization with Env-CaP-p30 than mice not vaccinated against Tetanus. The enhancing effect of ISH on anti-Env antibody levels was not attended with increased Env-specific IFN-γ CD4 T-cell responses that otherwise may potentially influence the susceptibility to HIV-1 infection. Thus, CaP nanoparticles functionalized with stabilized HIV-1 Env trimers and heterologous T-helper epitopes are able to recruit heterologous T-helper cells induced by a licensed vaccine and improve anti-Env antibody responses by intrastructural help.

Authors with CRIS profile

Dominik Damm Institute of Clinical and Molecular Virology Klaus Überla Institute of Clinical and Molecular Virology Vladimir Temchura Institute of Clinical and Molecular Virology

Involved external institutions

Universität Duisburg-Essen (UDE) DE Germany (DE) Scripps Research Institute (TSRI) US United States (USA) (US)

How to cite

APA:

Damm, D., Rojas-Sánchez, L., Theobald, H., Sokolova, V., Wyatt, R.T., Überla, K.,... Temchura, V. (2019). Calcium phosphate nanoparticle-based vaccines as a platform for improvement of HIV-1 env antibody responses by intrastructural help. Nanomaterials, 9(10). https://doi.org/10.3390/nano9101389

MLA:

Damm, Dominik, et al. "Calcium phosphate nanoparticle-based vaccines as a platform for improvement of HIV-1 env antibody responses by intrastructural help." Nanomaterials 9.10 (2019).

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