Effect of Tff3 Deficiency and ER Stress in the Liver

Šešelja K, Bazina I, Welss J, Schicht M, Paulsen F, Bijelić N, Rođak E, Horvatić A, Gelemanović A, Mihalj M, Baus Lončar M (2019)

Publication Type: Journal article

Publication year: 2019

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 20

Journal Issue: 18

DOI: 10.3390/ijms20184389

Abstract

Endoplasmic reticulum (ER) stress, a cellular condition caused by the accumulation of unfolded proteins inside the ER, has been recognized as a major pathological mechanism in a variety of conditions, including cancer, metabolic and neurodegenerative diseases. Trefoil factor family (TFFs) peptides are present in different epithelial organs, blood supply, neural tissues, as well as in the liver, and their deficiency has been linked to the ER function. Complete ablation of Tff3 expression is observed in steatosis, and as the most prominent change in the early phase of diabetes in multigenic mouse models of diabesity. To elucidate the role of Tff3 deficiency on different pathologically relevant pathways, we have developed a new congenic mouse model Tff3-/-/C57BL6/N from a mixed background strain (C57BL6/N /SV129) by using a speed congenics approach. Acute ER stress was evoked by tunicamycin treatment, and mice were sacrificed after 24 h. Afterwards the effect of Tff3 deficiency was evaluated with regard to the expression of relevant oxidative and ER stress genes, relevant proinflammatory cytokines/chemokines, and the global protein content. The most dramatic change was noticed at the level of inflammation-related genes, while markers for unfolded protein response were not significantly affected. Ultrastructural analysis confirmed that the size of lipid vacuoles was affected as well. Since the liver acts as an important metabolic and immunological organ, the influence of Tff3 deficiency and physiological function possibly reflects on the whole organism.

Authors with CRIS profile

Jessica Farger Lehrstuhl für Funktionelle und Klinische Anatomie Martin Schicht Lehrstuhl für Funktionelle und Klinische Anatomie Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie

Involved external institutions

Rudjer Boskovic Institute (RBI) / Institut Ruđer Bošković (IRB) HR Croatia (HR) Josip-Juraj-Strossmayer-Universität Osijek HR Croatia (HR) University of Zagreb / Sveučilište u Zagrebu HR Croatia (HR)

How to cite

APA:

Šešelja, K., Bazina, I., Welss, J., Schicht, M., Paulsen, F., Bijelić, N.,... Baus Lončar, M. (2019). Effect of Tff3 Deficiency and ER Stress in the Liver. International Journal of Molecular Sciences, 20(18). https://doi.org/10.3390/ijms20184389

MLA:

Šešelja, Kate, et al. "Effect of Tff3 Deficiency and ER Stress in the Liver." International Journal of Molecular Sciences 20.18 (2019).

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