Patient-derived cytomegaloviruses with different ganciclovir sensitivities from UL97 mutation retain their replication efficiency and some kinase activity in vitro

Wong DD, Van Zuylen WJ, Hamilton ST, Steingruber M, Sonntag E, Marschall M, Rawlinson WD (2019)


Publication Type: Journal article

Publication year: 2019

Journal

Book Volume: 63

Article Number: e02425-18

Journal Issue: 9

DOI: 10.1128/AAC.02425-18

Abstract

Mutations in the cytomegalovirus UL97 kinase gene contribute to antiviral resistance. Mutations A594S and G598D from two clinical isolates were analyzed, and bacterial artificial chromosome (BAC)-engineered A594S recombinant cytomegalovirus exhibited a ganciclovir-resistant phenotype on plaque reduction. Viral replication was comparable to that of the wild type. Cell-based kinase activity and autophosphorylation of ectopically expressed proteins showed that mutants retained some kinase activity. This study showed that patient-derived cytomegalovirus with different ganciclovir sensitivities retained replication efficiency and exhibited some kinase activity in vitro.

Authors with CRIS profile

Involved external institutions

How to cite

APA:

Wong, D.D., Van Zuylen, W.J., Hamilton, S.T., Steingruber, M., Sonntag, E., Marschall, M., & Rawlinson, W.D. (2019). Patient-derived cytomegaloviruses with different ganciclovir sensitivities from UL97 mutation retain their replication efficiency and some kinase activity in vitro. Antimicrobial Agents and Chemotherapy, 63(9). https://dx.doi.org/10.1128/AAC.02425-18

MLA:

Wong, Diana D., et al. "Patient-derived cytomegaloviruses with different ganciclovir sensitivities from UL97 mutation retain their replication efficiency and some kinase activity in vitro." Antimicrobial Agents and Chemotherapy 63.9 (2019).

BibTeX: Download