14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity

Rivero G, Aldana AA, Frontini Lopez YR, Liverani L, Boccaccini AR, Bustos DM, Abraham GA (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Journal of Materials Science: Materials in Medicine Springer

Book Volume: 30

Article Number: 99

Journal Issue: 9

DOI: 10.1007/s10856-019-6302-2

Abstract

Adipose-derived mesenchymal stem cells (ASCs) accelerate the osteointegration of bone grafts and improve the efficiency in the formation of uniform bone tissue, providing a practical and clinically attractive approach in bone tissue regeneration. In this work, the effect of nanofibrous biomimetic matrices composed of poly(ε-caprolactone) (PCL), nanometric hydroxyapatite (nHA) particles and 14-3-3 protein isoform epsilon on the initial stages of human ASCs (hASCs) osteogenic differentiation was investigated. The cells were characterized by flow cytometry and induction to differentiation to adipogenic and osteogenic lineages. The isolated hASCs were induced to differentiate to osteoblasts over all scaffolds, and adhesion and viability of the hASCs were found to be similar. However, the activity of alkaline phosphatase (ALP) as early osteogenic marker in the PCL-nHA/protein scaffold was four times higher than in PCL-nHA and more than five times than the measured in neat PCL. [Figure not available: see fulltext.].

Authors with CRIS profile

Liliana Liverani Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Instituto de Investigaciones en Ciencia y Tecnología de Materiales (INTEMA) AR Argentina (AR) Laboratorio de Integración de Señales Celulares AR Argentina (AR)

How to cite

APA:

Rivero, G., Aldana, A.A., Frontini Lopez, Y.R., Liverani, L., Boccaccini, A.R., Bustos, D.M., & Abraham, G.A. (2019). 14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity. Journal of Materials Science: Materials in Medicine, 30(9). https://dx.doi.org/10.1007/s10856-019-6302-2

MLA:

Rivero, G., et al. "14-3-3ε protein-immobilized PCL-HA electrospun scaffolds with enhanced osteogenicity." Journal of Materials Science: Materials in Medicine 30.9 (2019).

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