Establishment of reference values for the lysine acetylation marker N ɛ-acetyllysine in small volume human plasma samples by a multi-target LC–MS/MS method

Journal article


Publication Details

Author(s): Geßner A, Mieth M, Auge D, Chafai A, Müller F, Fromm M, Maas R
Journal: Amino Acids
Publication year: 2019
ISSN: 0939-4451


Abstract

Cardiovascular disease (CVD) and chronic kidney disease (CKD) constitute substantial burdens for public health. The identification and validation of risk markers for CVD and CKD in epidemiological studies requires frequent adaption of existing analytical methods as well as development of new methods. In this study, an analytical procedure to simultaneously quantify ten endogenous biomarkers for CVD and CKD is described. An easy-to-handle sample preparation requiring only 20 µL of human plasma is followed by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). The method was successfully validated according to established guidelines meeting required criteria for accuracy, precision, recovery, linearity, selectivity, and limits of quantification. The scalability of the method for application in larger cohorts was assessed using a set of plasma samples from healthy volunteers (n = 391) providing first reference values for the recently established biomarker Nɛ-acetyllysine (Nɛ-AcLys). Other biomarkers analyzed were creatinine, β-aminoisobutyric acid (β-AIB), carnitine, 1-methylnicotinamide (1-MNA), citrulline, symmetric dimethylarginine (SDMA), asymmetric dimethylarginine (ADMA), homoarginine (hArg), and ornithine. All obtained results are within reference values specified elsewhere. Overall, these results demonstrate the suitability of the method for simultaneous quantification of ten endogenous biomarkers for CVD and CKD in plasma samples from larger cohorts and allow validation of Nɛ-AcLys as a biomarker in large cohorts.


FAU Authors / FAU Editors

Auge, Daniel
Lehrstuhl für Klinische Pharmakologie und Klinische Toxikologie
Chafai, Anja Dr.
Lehrstuhl für Klinische Pharmakologie und Klinische Toxikologie
Fromm, Martin Prof. Dr.
Lehrstuhl für Klinische Pharmakologie und Klinische Toxikologie
Geßner, Arne Dr.
Lehrstuhl für Klinische Pharmakologie und Klinische Toxikologie
Maas, Renke Prof. Dr.
Professur für Klinische Pharmakologie
Mieth, Maren Dr.
Lehrstuhl für Klinische Pharmakologie und Klinische Toxikologie
Müller, Fabian Dr.
Lehrstuhl für Klinische Pharmakologie und Klinische Toxikologie


How to cite

APA:
Geßner, A., Mieth, M., Auge, D., Chafai, A., Müller, F., Fromm, M., & Maas, R. (2019). Establishment of reference values for the lysine acetylation marker N ɛ-acetyllysine in small volume human plasma samples by a multi-target LC–MS/MS method. Amino Acids. https://dx.doi.org/10.1007/s00726-019-02765-8

MLA:
Geßner, Arne, et al. "Establishment of reference values for the lysine acetylation marker N ɛ-acetyllysine in small volume human plasma samples by a multi-target LC–MS/MS method." Amino Acids (2019).

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Last updated on 2019-14-08 at 08:23