Choroid plexus-derived miR-204 regulates the number of quiescent neural stem cells in the adult brain
Lepko T, Pusch M, Mueller T, Schulte D, Ehses J, Kiebler M, Hasler J, Huttner H, Vandenbroucke RE, Vandendriessche C, Modic M, Martin-Villalba A, Zhao S, Llorens-Bobadilla E, Schneider A, Fischer A, Breunig CT, Stricker SH, Goetz M, Ninkovic J (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Abstract
Regulation of adult neural stem cell (NSC) number is critical for lifelong neurogenesis. Here, we identified a post-transcriptional control mechanism, centered around the microRNA 204 (miR-204), to control the maintenance of quiescent (q)NSCs. miR-204 regulates a spectrum of transcripts involved in cell cycle regulation, neuronal migration, and differentiation in qNSCs. Importantly, inhibition of miR-204 function reduced the number of qNSCs in the subependymal zone (SEZ) by inducing pre-mature activation and differentiation of NSCs without changing their neurogenic potential. Strikingly, we identified the choroid plexus of the mouse lateral ventricle as the major source of miR-204 that is released into the cerebrospinal fluid to control number of NSCs within the SEZ. Taken together, our results describe a novel mechanism to maintain adult somatic stem cells by a niche-specific miRNA repressing activation and differentiation of stem cells.
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Involved external institutions
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Flanders Institute for Biotechnology / Vlaams Instituut voor Biotechnologie (VIB)
Belgium (BE)
Deutsches Krebsforschungszentrum (DKFZ)
Germany (DE)
The Francis Crick Institute
United Kingdom (GB)
Goethe-Universität Frankfurt am Main
Germany (DE)
Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Germany (DE)
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Flanders Institute for Biotechnology / Vlaams Instituut voor Biotechnologie (VIB)
Belgium (BE)
Deutsches Krebsforschungszentrum (DKFZ)
Germany (DE)
The Francis Crick Institute
United Kingdom (GB)
Goethe-Universität Frankfurt am Main
Germany (DE)
Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Germany (DE)
How to cite
APA:
Lepko, T., Pusch, M., Mueller, T., Schulte, D., Ehses, J., Kiebler, M.,... Ninkovic, J. (2019). Choroid plexus-derived miR-204 regulates the number of quiescent neural stem cells in the adult brain. EMBO Journal. https://dx.doi.org/10.15252/embj.2018100481
MLA:
Lepko, Tjasa, et al. "Choroid plexus-derived miR-204 regulates the number of quiescent neural stem cells in the adult brain." EMBO Journal (2019).
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