Deciphering the genetic basis for vitamin E accumulation in leaves and grains of different barley accessions

Journal article


Publication Details

Author(s): Schuy C, Groth J, Ammon A, Eydam J, Baier S, Schweizer G, Hanemann A, Herz M, Voll L, Sonnewald U
Journal: Scientific Reports
Publication year: 2019
Volume: 9
Journal issue: 1
ISSN: 2045-2322


Abstract

Tocopherols and tocotrienols, commonly referred to as vitamin E, are essential compounds in food and feed. Due to their lipophilic nature they protect biomembranes by preventing the propagation of lipid-peroxidation especially during oxidative stress. Since their synthesis is restricted to photosynthetic organisms, plant-derived products are the major source of natural vitamin E. In the present study the genetic basis for high vitamin E accumulation in leaves and grains of different barley (Hordeum vulgare L.) accessions was uncovered. A genome wide association study (GWAS) allowed the identification of two genes located on chromosome 7H, homogentisate phytyltransferase (HPT-7H) and homogentisate geranylgeranyltransferase (HGGT) that code for key enzymes controlling the accumulation of tocopherols in leaves and tocotrienols in grains, respectively. Transcript profiling showed a correlation between HPT-7H expression and vitamin E content in leaves. Allele sequencing allowed to decipher the allelic variation of HPT-7H and HGGT genes corresponding to high and low vitamin E contents in the respective tissues. Using the obtained sequence information molecular markers have been developed which can be used to assist smart breeding of high vitamin E barley varieties. This will facilitate the selection of genotypes more tolerant to oxidative stress and producing high-quality grains.


FAU Authors / FAU Editors

Ammon, Alexandra
Lehrstuhl für Biochemie
Baier, Steffen
Lehrstuhl für Biochemie
Eydam, Julia
Lehrstuhl für Biochemie
Schuy, Christian
Lehrstuhl für Biochemie
Sonnewald, Uwe Prof. Dr.
Lehrstuhl für Biochemie
Voll, Lars PD Dr.
Lehrstuhl für Biochemie


External institutions with authors

Bayerische Landesanstalt für Landwirtschaft
Saatzucht Josef Breun GmbH & Co. KG


How to cite

APA:
Schuy, C., Groth, J., Ammon, A., Eydam, J., Baier, S., Schweizer, G.,... Sonnewald, U. (2019). Deciphering the genetic basis for vitamin E accumulation in leaves and grains of different barley accessions. Scientific Reports, 9(1). https://dx.doi.org/10.1038/s41598-019-45572-7

MLA:
Schuy, Christian, et al. "Deciphering the genetic basis for vitamin E accumulation in leaves and grains of different barley accessions." Scientific Reports 9.1 (2019).

BibTeX: 

Last updated on 2019-22-07 at 07:33