Gpr126 (Adgrg6) is expressed in cell types known to be exposed to mechanical stimuli

Musa G, Cazorla-Vázquez S, van Amerongen MJ, Stemmler M, Eckstein M, Hartmann A, Braun T, Brabletz T, Engel F (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Annals of the New York Academy of Sciences New York Academy of Sciences

Publisher: Blackwell Publishing Inc.

DOI: 10.1111/nyas.14135

Abstract

GPR126 (ADGRG6) is an adhesion G protein–coupled receptor that plays an important role in a variety of tissues/organs, such as heart, sciatic nerve, cartilage, and ear. Moreover, GPR126 (ADGRG6) mutations are associated with human diseases, like adolescent idiopathic scoliosis, lung disease, bladder cancer, and intellectual disability. Despite its clinical importance, it remains elusive how GPR126 is activated and mediates signal transduction and what cellular processes depend on GPR126 signaling. Here, we generated a lacZ reporter mouse line to determine endogenous Gpr126 (Adgrg6) expression in a cell type-specific manner during embryonic development, at postnatal day 5 and in adult animals. Our results confirm Gpr126 expression data previously obtained utilizing antibodies and in situ hybridization in embryonic heart and sciatic nerve. In addition, we provide data with cellular resolution for previously described RT-PCR–based data, including lung and bladder. Moreover, new Gpr126-expressing tissues and cell types were identified, such as ureter and acinar secretory cells. Collectively, our data demonstrate that the newly generated lacZ reporter mouse is a suitable model to study Gpr126 expression during development and adulthood, provide detailed insight into Gpr126 expression at the cellular level, and reveal that all identified Gpr126-expressing cells are known to be exposed to mechanical stimuli.

Authors with CRIS profile

Gentian Musa Department of Nephropathology Marc Stemmler Lehrstuhl für Experimentelle Medizin mit dem Schwerpunkt Molekulare Pathogeneseforschung Markus Eckstein Institute of Pathology Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Thomas Brabletz Lehrstuhl für Experimentelle Medizin mit dem Schwerpunkt Molekulare Pathogeneseforschung Felix Engel Professur für Experimentelle Nieren- und Kreislaufforschung

Involved external institutions

Max Planck Institute for Heart and Lung Research / Max-Planck-Institut für Herz- und Lungenforschung DE Germany (DE)

How to cite

APA:

Musa, G., Cazorla-Vázquez, S., van Amerongen, M.J., Stemmler, M., Eckstein, M., Hartmann, A.,... Engel, F. (2019). Gpr126 (Adgrg6) is expressed in cell types known to be exposed to mechanical stimuli. Annals of the New York Academy of Sciences. https://doi.org/10.1111/nyas.14135

MLA:

Musa, Gentian, et al. "Gpr126 (Adgrg6) is expressed in cell types known to be exposed to mechanical stimuli." Annals of the New York Academy of Sciences (2019).

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