Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities

Journal article


Publication Details

Author(s): Mukherjee S, Ghosh K, Hahn F, Wangen C, Strojan H, Müller R, Anand N, Ali I, Bera K, Ray B, Hutterer C, Marschall M, Ray S
Journal: International Journal of Biological Macromolecules
Publication year: 2019
Volume: 136
Pages range: 521-530
ISSN: 0141-8130


Abstract

The provisioning of compound libraries with a high degree of diversity and attractive pharmacological properties is a limiting step in drug development. This study reports the production of highly bioactive sulfated polysaccharides, originally present in a nonsulfated, dormant state in natural sources, and demonstrates their antiviral activity (human cytomegalovirus EC50 values of 2.34–7.77 μg/mL) at a low degree of cytotoxicity. Furthermore, data strongly suggested the inhibition of virus entry as the main mode of antiviral action. Remarkably, the utilized oleum-DMF reagent was able to generate a range of sulfated polysaccharides from various natural sources, possessing varying saccharide compositions, degrees of sulfation (0.4–1.7) and molecular masses (38–94,000 g/mol). Typically, in a matter of minutes, this reagent not only solubilized polysaccharides but also chemically converted their hydroxyl functionality into sulfates. The most active sulfated polysaccharide (EC50 of 2.62 μg/mL) proved to be a 94,000 g/mol branched glucan with sulfates at C-6/C-3,6/C-2,3,6 positions. In conclusion, the important determinants of such compounds' antiviral activity are: (i) degree of sulfation, (ii) molecular mass and (iii) structural features. Thus, our approach offers a huge prospect for the improvement of natural source-derived libraries based on biologically active polysaccharides with diversified chemical profiles.


FAU Authors / FAU Editors

Hahn, Friedrich
Professur für Virologie


External institutions with authors

The University of Burdwan


How to cite

APA:
Mukherjee, S., Ghosh, K., Hahn, F., Wangen, C., Strojan, H., Müller, R.,... Ray, S. (2019). Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities. International Journal of Biological Macromolecules, 136, 521-530. https://dx.doi.org/10.1016/j.ijbiomac.2019.05.005

MLA:
Mukherjee, Shuvam, et al. "Chemically sulfated polysaccharides from natural sources: Assessment of extraction-sulfation efficiencies, structural features and antiviral activities." International Journal of Biological Macromolecules 136 (2019): 521-530.

BibTeX: 

Last updated on 2019-01-07 at 09:23