Contribution of LTi and T(H)17 cells to B cell aggregate formation in the central nervous system in a mouse model of multiple sclerosis

Journal article


Publication Details

Author(s): Schropp V, Rohde J, Rovituso DM, Jabari S, Bharti R, Kürten S
Journal: Journal of Neuroinflammation
Publication year: 2019
Volume: 16
ISSN: 1742-2094


Abstract

BackgroundIn a subgroup of patients suffering from progressive multiple sclerosis (MS), which is an inflammation-mediated neurodegenerative disease of the central nervous system (CNS), B cell aggregates were discovered within the meninges. Occurrence of these structures was associated with a more severe disease course and cortical histopathology. We have developed the B cell-dependent MP4-induced experimental autoimmune encephalomyelitis (EAE) as a mouse model to mimic this trait of the human disease. The aim of this study was to determine a potential role of lymphoid tissue inducer (LTi) and T(H)17 cells in the process of B cell aggregate formation in the MP4 model.MethodsWe performed flow cytometry of cerebellar and splenic tissue of MP4-immunized mice in the acute and chronic stage of the disease to analyze the presence of CD3(-)CD5(-)CD4(+)RORt(+) LTi and CD3(+)CD5(+)CD4(+)RORt(+) T(H)17 cells. Myelin oligodendrocyte glycoprotein (MOG):35-55-induced EAE was used as B cell-independent control model. We further determined the gene expression profile of B cell aggregates using laser capture microdissection, followed by RNA sequencing.ResultsWhile we were able to detect LTi cells in the embryonic spleen and adult intestine, which served as positive controls, there was no evidence for the existence of such a population in acute or chronic EAE in neither of the two models. Yet, we detected CD3(-)CD5(-)CD4(-)RORt(+) innate lymphoid cells (ILCs) and T(H)17 cells in the CNS, the latterespecially in the chronic stage of MP4-induced EAE. Moreover, we observed a unique gene signature in CNS B cell aggregates compared to draining lymph nodes of MP4-immunized mice and to cerebellum as well as draining lymph nodes of mice with MOG:35-55-induced EAE.ConclusionThe absence of LTi cells in the cerebellum suggests that other cells might take over the function as an initiator of lymphoid tissue formation in the CNS. Overall, the development of ectopic lymphoid organs is a complex process based on an interplay between several molecules and signals. Here, we propose some potential candidates, which might be involved in the formation of B cell aggregates in the CNS of MP4-immunized mice.


FAU Authors / FAU Editors

Jabari, Samir PD Dr.
Lehrstuhl für Anatomie und Zellbiologie
Kürten, Stefanie Prof. Dr. med.
Lehrstuhl für Anatomie und Zellbiologie
Schropp, Verena
Lehrstuhl für Anatomie und Zellbiologie


External institutions with authors

Julius-Maximilians-Universität Würzburg
Universitätsklinikum Würzburg


How to cite

APA:
Schropp, V., Rohde, J., Rovituso, D.M., Jabari, S., Bharti, R., & Kürten, S. (2019). Contribution of LTi and T(H)17 cells to B cell aggregate formation in the central nervous system in a mouse model of multiple sclerosis. Journal of Neuroinflammation, 16. https://dx.doi.org/10.1186/s12974-019-1500-x

MLA:
Schropp, Verena, et al. "Contribution of LTi and T(H)17 cells to B cell aggregate formation in the central nervous system in a mouse model of multiple sclerosis." Journal of Neuroinflammation 16 (2019).

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Last updated on 2019-20-06 at 03:08