Detection and virulence potential of a phospholipase D-negative Corynebacterium ulcerans from a concurrent diphtheria and infectious mononucleosis case

Simpson-Lourêdo L, Silva CM, Hacker E, Souza NF, Santana MM, Antunes CA, Nagao PE, Hirata R, Burkovski A, Villas Bôas MHS, Mattos-Guaraldi AL (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Springer Verlag (Germany)

Book Volume: 112

Pages Range: 1055-1065

Journal Issue: 7

DOI: 10.1007/s10482-019-01240-4

Abstract

Diphtheria by Corynebacterium ulcerans is increasingly occurring in children, adolescents and adults. In addition to diphtheria toxin (DT), phospholipase D (PLD) is considered a virulence factor of C. ulcerans. In the present study, a first case of concurrent diphtheria by a PLD-negative C. ulcerans and infectious mononucleosis (IM) was verified. Clinical and microbiological profiles and binding properties to human Fibrinogen (Fbg), Fibronectin (Fn) and type I collagen (col I) biotinylated proteins and virulence to Caenorhabditis elegans were investigated for C. ulcerans strain 2590 (clinical isolate) and two control strains, including PLD-positive BR-AD22 wild type and PLD-negative ELHA-1 PLD mutant strains. MALDI-TOF assays and a multiplex PCR of genes coding for potentially toxigenic corynebacteria identified strain 2590 as non-DT producing. Interestingly, strain 2590 did not express PLD activity in the CAMP test although the presence of the pld gene was verified. PLD-negative 2590 and a PLD-positive 210932 strains showed similar affinity to Fbg, Fn and type I collagen. C. elegans were able to escape from C. ulcerans strains, independent of PLD and DT production. Higher mortality of nematodes was verified for PLD-negative strains. Additional studies concerning multifactorial virulence potential of C. ulcerans, including environmental conditions remain necessary.

Authors with CRIS profile

Elena Hacker Professur für Mikrobiologie

Involved external institutions

Rio de Janeiro State University / Universidade do Estado do Rio de Janeiro (UERJ) BR Brazil (BR) Oswaldo Cruz Foundation / Fundação Oswaldo Cruz (FIOCRUZ) BR Brazil (BR)

How to cite

APA:

Simpson-Lourêdo, L., Silva, C.M., Hacker, E., Souza, N.F., Santana, M.M., Antunes, C.A.,... Mattos-Guaraldi, A.L. (2019). Detection and virulence potential of a phospholipase D-negative Corynebacterium ulcerans from a concurrent diphtheria and infectious mononucleosis case. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 112(7), 1055-1065. https://dx.doi.org/10.1007/s10482-019-01240-4

MLA:

Simpson-Lourêdo, Liliane, et al. "Detection and virulence potential of a phospholipase D-negative Corynebacterium ulcerans from a concurrent diphtheria and infectious mononucleosis case." Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 112.7 (2019): 1055-1065.

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