Tri-layered functionally graded membrane for potential application in periodontal regeneration

Journal article

Publication Details

Author(s): Shah AT, Zahid S, Ikram F, Maqbool M, Chaudhry AA, Rahim MI, Schmidt F, Goerke O, Khan AS, Rehman Iu
Journal: Materials Science and Engineering: C
Publication year: 2019
Volume: 103
ISSN: 0928-4931
eISSN: 1873-0191


A novel tri-layered, functionally-graded chitosan membrane (FGM) with bioactive glass gradient (50%, 25%, and 0% wt.) was developed by lyophilization. A step-wise grading of chitosan, bioactive glass (BG), and Pluronic F127 was introduced into the membrane in which each layer has separate surface functions that play a role of guided tissue regeneration (GTR) membranes. The lower layer was designed to replicate alveolar bone and contains 50%wt. BG, the middle layer contains 25%wt. BG, while the upper layer was non-porous without BG and it did not support cell growth. Scanning Electron Microscopy (SEM) revealed that the lower FGM surface possessed a porous structure with embedded BG particles, while the upper surface was non-porous with interconnected architecture. The contact angle measurement confirmed that the surface with BG was hydrophilic (≈00), while the opposite surface was hydrophobic (910 ± 3.840). Both osteoblast and fibroblast cells have maximum adhesion at contact angle <80°. Alamar blue assay revealed the biocompatibility of the MC3T3-E1 mouse pre-osteoblasts cells with these membranes in vitro. The cells attachment and proliferation was seen for lower surface, while no cells adhesion was observed for the upper layer. Additionally, the interaction of the tissue with these tri-layered membranes was also investigated in vivo. Hematoxylin and eosin staining revealed the biocompatible nature of these membranes. Altogether, these results indicated that due to the biocompatible nature of these membranes, they will be a good carrier of in vivo implantation.

FAU Authors / FAU Editors

Maqbool, Muhammad
Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

External institutions with authors

COMSATS Institute of Information Technology
Imam Abdulrahman Bin Faisal University
Technische Universität Berlin
University of Sheffield

How to cite

Shah, A.T., Zahid, S., Ikram, F., Maqbool, M., Chaudhry, A.A., Rahim, M.I.,... Rehman, I.u. (2019). Tri-layered functionally graded membrane for potential application in periodontal regeneration. Materials Science and Engineering: C, 103.

Shah, Asma Tufail, et al. "Tri-layered functionally graded membrane for potential application in periodontal regeneration." Materials Science and Engineering: C 103 (2019).


Last updated on 2019-20-06 at 00:08