Quantitative synchrotron X-ray tomography of the material-tissue interface in rat cortex implanted with neural probes

Böhm T, Joseph K, Kirsch M, Moroni R, Hilger A, Osenberg M, Manke I, Johnston M, Stieglitz T, Hofmann UG, Haas CA, Thiele S (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 9

Article Number: 7646

Journal Issue: 1

DOI: 10.1038/s41598-019-42544-9

Abstract

Neural probes provide many options for neuroscientific research and medical purposes. However, these implantable micro devices are not functionally stable over time due to host-probe interactions. Thus, reliable high-resolution characterization methods are required to understand local tissue changes upon implantation. In this work, synchrotron X-ray tomography is employed for the first time to image the interface between brain tissue and an implanted neural probe, showing that this 3D imaging method is capable of resolving probe and surrounding tissue at a resolution of about 1 micrometer. Unstained tissue provides sufficient contrast to identify electrode sites on the probe, cells, and blood vessels within tomograms. Exemplarily, we show that it is possible to quantify characteristics of the interaction region between probe and tissue, like the blood supply system. Our first-time study demonstrates a way for simultaneous 3D investigation of brain tissue with implanted probe, providing information beyond what was hitherto possible.

Authors with CRIS profile

Simon Thiele Professur für Electrocatalytic Interface Engineering (HIERN)

Involved external institutions

Albert-Ludwigs-Universität Freiburg DE Germany (DE) Helmholtz-Zentrum Berlin für Materialien und Energie (HZB) DE Germany (DE)

How to cite

APA:

Böhm, T., Joseph, K., Kirsch, M., Moroni, R., Hilger, A., Osenberg, M.,... Thiele, S. (2019). Quantitative synchrotron X-ray tomography of the material-tissue interface in rat cortex implanted with neural probes. Scientific Reports, 9(1). https://dx.doi.org/10.1038/s41598-019-42544-9

MLA:

Böhm, Thomas, et al. "Quantitative synchrotron X-ray tomography of the material-tissue interface in rat cortex implanted with neural probes." Scientific Reports 9.1 (2019).

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