Direct Zinc Finger Protein Persulfidation by H2S Is Facilitated by Zn2+

Lange M, Ok K, Shimberg GD, Bursac B, Markó L, Ivanovic-Burmazovic I, Michel SL, Filipovic M (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Angewandte Chemie International Edition Wiley

DOI: 10.1002/anie.201900823

Abstract

H 2 S is a gaseous signaling molecule that modifies cysteine residues in proteins to form persulfides (P-SSH). One family of proteins modified by H 2 S are zinc finger (ZF) proteins, which contain multiple zinc-coordinating cysteine residues. Herein, we report the reactivity of H 2 S with a ZF protein called tristetraprolin (TTP). Rapid persulfidation leading to complete thiol oxidation of TTP mediated by H 2 S was observed by low-temperature ESI-MS and fluorescence spectroscopy. Persulfidation of TTP required O 2 , which reacts with H 2 S to form superoxide, as detected by ESI-MS, a hydroethidine fluorescence assay, and EPR spin trapping. H 2 S was observed to inhibit TTP function (binding to TNFα mRNA) by an in vitro fluorescence anisotropy assay and to modulate TNFα in vivo. H 2 S was unreactive towards TTP when the protein was bound to RNA, thus suggesting a protective effect of RNA.

Authors with CRIS profile

Ivana Ivanovic-Burmazovic Lehrstuhl für Bioanorganische Chemie Milos Filipovic Lehrstuhl für Bioanorganische Chemie

Involved external institutions

University of Maryland School of Pharmacy US United States (USA) (US) Université de Bordeaux FR France (FR) Max-Delbrück-Centrum für Molekulare Medizin / Max Delbrück Center for Molecular Medicine (MDC) Berlin-Buch DE Germany (DE)

How to cite

APA:

Lange, M., Ok, K., Shimberg, G.D., Bursac, B., Markó, L., Ivanovic-Burmazovic, I.,... Filipovic, M. (2019). Direct Zinc Finger Protein Persulfidation by H2S Is Facilitated by Zn2+. Angewandte Chemie International Edition. https://dx.doi.org/10.1002/anie.201900823

MLA:

Lange, Mike, et al. "Direct Zinc Finger Protein Persulfidation by H2S Is Facilitated by Zn2+." Angewandte Chemie International Edition (2019).

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