Transcriptome and histone epigenome of Plasmodium vivax salivary-gland sporozoites point to tight regulatory control and mechanisms for liver-stage differentiation in relapsing malaria

Journal article


Publication Details

Author(s): Muller I, Jex AR, Kappe SH, Mikolajczak SA, Sattabongkot J, Patrapuvich R, Lindner S, Flannery EL, Koepfli C, Ansell B, Lerch A, Emery-Corbin SJ, Charnaud S, Smith J, Merrienne N, Swearingen KE, Moritz RL, Petter M, Duffy MF, Chuenchob V
Journal: International Journal For Parasitology
Publication year: 2019
ISSN: 0020-7519


Abstract

Plasmodium vivax is the key obstacle to malaria elimination in Asia and Latin America, largely attributed to its ability to form resilient hypnozoites (sleeper cells) in the host liver that escape treatment and cause relapsing infections. The decision to form hypnozoites is made early in the liver infection and may already be set in sporozoites prior to invasion. To better understand these early stages of infection, we undertook a comprehensive transcriptomic and histone epigenetic characterization of P. vivax sporozoites. Through comparisons with recently published proteomic data for the P. vivax sporozoite, our study found that although highly transcribed, transcripts associated with functions needed for early infection of the vertebrate host are not detectable as proteins and may be regulated through translational repression. We identified differential transcription between the sporozoite and published transcriptomes of asexual blood stages and mixed versus hypnozoite-enriched liver stages. These comparisons point to multiple layers of transcriptional, post-transcriptional and post-translational control that appear active in sporozoites and to a lesser extent hypnozoites, but are largely absent in replicating liver schizonts or mixed blood stages. We also characterised histone epigenetic modifications in the P. vivax sporozoite and explored their role in regulating transcription. Collectively, these data support the hypothesis that the sporozoite is a tightly programmed stage to infect the human host and identify mechanisms for hypnozoite formation that may be further explored in liver stage models.


FAU Authors / FAU Editors

Petter, Michaela Dr. rer. nat.
Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene


External institutions with authors

Institute for Systems Biology
Institut Pasteur
Mahidol University / มหาวิทยาลัยมหิดล
Pennsylvania State University (Penn State)
Seattle Children's Hospital
The University of Melbourne
University of Washington
Walter and Eliza Hall Institute of Medical Research (WEHI)


How to cite

APA:
Muller, I., Jex, A.R., Kappe, S.H., Mikolajczak, S.A., Sattabongkot, J., Patrapuvich, R.,... Chuenchob, V. (2019). Transcriptome and histone epigenome of Plasmodium vivax salivary-gland sporozoites point to tight regulatory control and mechanisms for liver-stage differentiation in relapsing malaria. International Journal For Parasitology. https://dx.doi.org/10.1016/j.ijpara.2019.02.007

MLA:
Muller, Ivo, et al. "Transcriptome and histone epigenome of Plasmodium vivax salivary-gland sporozoites point to tight regulatory control and mechanisms for liver-stage differentiation in relapsing malaria." International Journal For Parasitology (2019).

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Last updated on 2019-23-05 at 08:08