Microvesicles from cerebrospinal fluid of patients with Alzheimer’s disease display reduced concentrations of tau and APP protein

Spitzer P, Mulzer LM, Oberstein T, Munoz LE, Lewczuk P, Kornhuber J, Herrmann M, Maler JM (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 9

Article Number: 7089

Journal Issue: 1

DOI: 10.1038/s41598-019-43607-7

Abstract

Microvesicles are small membranous particles generated during cellular activation or stress. The analysis of the content and the surface of microvesicles allow conclusions about the cells they are originating from and the underlying pathology. Therefore, CSF microvesicles have been suggested to be promising targets to monitor the (etio)pathology of neurodegenerative diseases. Microvesicles in the CSF of 15 patients with Alzheimer’s disease and 15 controls were analyzed by flow cytometry regarding the levels of CD3, CD4, CD45, CD64, BACE1, Aβ, APP and tau. The results were replicated in a second cohort comprising 14 patients with Alzheimer’s disease and 9 controls. The levels of tau and APP were reduced in microvesicles of Alzheimer’s disease patients. A significant change was neither observed in the number of microvesicles nor in the expression of the other antigens. Tau and APP in microvesicles separated patients with Alzheimer’s disease from controls with an AUC of 0.84 and 0.89 respectively. We conclude that tau and APP in CSF microvesicles are promising biomarkers which could directly provide information about the Alzheimer pathology on a cellular level.

Authors with CRIS profile

Philipp Spitzer Department of Psychiatry and Psychotherapy Linda-Marie Mulzer Department of Paediatrics and Adolescent Medicine Timo Oberstein Medizinische Fakultät Luis Munoz Becerra Department of Medicine 3 – Rheumatology and Immunology Piotr Lewczuk Department of Psychiatry and Psychotherapy Johannes Kornhuber Lehrstuhl für Psychiatrie und Psychotherapie Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology

How to cite

APA:

Spitzer, P., Mulzer, L.-M., Oberstein, T., Munoz, L.E., Lewczuk, P., Kornhuber, J.,... Maler, J.M. (2019). Microvesicles from cerebrospinal fluid of patients with Alzheimer’s disease display reduced concentrations of tau and APP protein. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-43607-7

MLA:

Spitzer, Philipp, et al. "Microvesicles from cerebrospinal fluid of patients with Alzheimer’s disease display reduced concentrations of tau and APP protein." Scientific Reports 9.1 (2019).

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