Innate extracellular vesicles from melanoma patients suppress beta-catenin in tumor cells by miRNA-34a

Lee JH, Dindorf J, Eberhardt M, Lai X, Ostalecki C, Koliha N, Gross S, Blume K, Bruns H, Wild S, Schuler G, Vera J, Baur A (2019)

Publication Type: Journal article, Original article

Publication year: 2019

Journal

Life Science Alliance Life Science Alliance

Book Volume: 2

Article Number: e201800205

Journal Issue: 2

DOI: 10.26508/lsa.201800205

Abstract

Upon tumor development, new extracellular vesicles appear in circulation. Our knowledge of their relative abundance, function, and overall impact on cancer development is still preliminary. Here, we demonstrate that plasma extracellular vesicles (pEVs) of non-tumor origin are persistently increased in untreated and post-excision melanoma patients, exhibiting strong suppressive effects on the proliferation of tumor cells. Plasma vesicle numbers, miRNAs, and protein levels were elevated two-to tenfold and detected many years after tumor resection. The vesicles revealed individual and clinical stage-specific miRNA profiles as well as active ADAM10. However, whereas pEV from patients preventing tumor relapse down-regulated beta-catenin and blocked tumor cell proliferation in an miR-34a-dependent manner, pEV from metastatic patients lost this ability and stimulated beta-catenin-mediated transcription. Cancer-induced pEV may constitute an innate immune mechanismsuppressing tumor cell activity including that of residual cancer cells present after primary surgery.

Authors with CRIS profile

Martin Eberhardt Lehrstuhl für Biochemie und Molekulare Medizin Xin Lai Department of Dermatology Heiko Bruns Department of Medicine 5 – Haematology and Oncology Gerold Schuler Lehrstuhl für Haut- und Geschlechtskrankheiten Julio Vera González Professur für Tumorimmunologie (Schwerpunkt Systembiologie)

Involved external institutions

Miltenyi Biotec GmbH DE Germany (DE)

How to cite

APA:

Lee, J.-H., Dindorf, J., Eberhardt, M., Lai, X., Ostalecki, C., Koliha, N.,... Baur, A. (2019). Innate extracellular vesicles from melanoma patients suppress beta-catenin in tumor cells by miRNA-34a. Life Science Alliance, 2(2). https://doi.org/10.26508/lsa.201800205

MLA:

Lee, Jung-Hyun, et al. "Innate extracellular vesicles from melanoma patients suppress beta-catenin in tumor cells by miRNA-34a." Life Science Alliance 2.2 (2019).

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