Magnetic Tissue Engineering of the Vocal Fold Using Superparamagnetic Iron Oxide Nanoparticles

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autorinnen und Autoren: Poettler M, Fliedner A, Bergmann J, Bui LK, Mühlberger M, Braun C, Graw M, Janko C, Friedrich O, Alexiou C, Lyer S
Zeitschrift: Tissue Engineering: Parts A, B, and C
Jahr der Veröffentlichung: 2019
ISSN: 1937-3368
eISSN: 1557-8690


Losing one's ability to speak, because of tissue deficiency at the vocal fold (VF), leads to serious impairment in the quality of life. Until now, there is no successful approach for regenerating the VF. The aim of this study was to show the advantage of magnetic nanoparticles in the generation of scaffold-free three-dimensional (3D) VF cell constructs by magnetic tissue engineering (MTE). Rabbit VF fibroblasts were used to establish MTE: after cellular uptake of superparamagnetic iron oxide nanoparticles (SPIONs), cells can be controlled with a magnetic field thereby forming solid 3D cell structures. To transfer this method into human cells, SPIONs were adapted accordingly and tested for their influence on human VF (hVF) cells and for their ability to perform MTE with hVF cells. Of interest, the cell number and the magnet's shape influence the form of the rabbit VF cell construct. After successful characterization of hVF cells, biocompatibility analyses revealed no significant influence of SPIONs on them, thus 3D hVF cell constructs could be successfully generated by MTE. These basic results are important to develop MTE as an innovative method to regenerate functional VFs. We expect that in vivo studies, including MTE as an elegant, far-field controlled and touchless technology, will translate MTE VF bioconstructs into reconstructive laryngeal medicine. Impact Statement This study aims at nanotechnology for regenerative medicine by magnetic tissue engineering (MTE). New approaches for vocal fold (VF) reconstruction are desperately needed. Superparamagnetic iron oxide nanoparticles offer innovative, scaffold-free potentials for tissue engineering: MTE. By using MTE we could generate functional multilayered human VF cell constructs, which can consequently be used to regenerate the voice in patients with VF injuries.

FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Alexiou, Christoph Prof. Dr.
Professur für Nanomedizin
Fliedner, Anna
Lehrstuhl für Humangenetik
Friedrich, Oliver Prof. Dr. Dr.
Lyer, Stefan Dr. rer. nat.
Lehrstuhl für Medizinische Biotechnologie
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Janko, Christina Dr. rer. nat.
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie
Mühlberger, Marina
Hals-Nasen-Ohren-Klinik - Kopf- und Halschirurgie

Einrichtungen weiterer Autorinnen und Autoren

Ludwig-Maximilians-Universität (LMU)


Poettler, M., Fliedner, A., Bergmann, J., Bui, L.K., Mühlberger, M., Braun, C.,... Lyer, S. (2019). Magnetic Tissue Engineering of the Vocal Fold Using Superparamagnetic Iron Oxide Nanoparticles. Tissue Engineering: Parts A, B, and C.

Poettler, Marina, et al. "Magnetic Tissue Engineering of the Vocal Fold Using Superparamagnetic Iron Oxide Nanoparticles." Tissue Engineering: Parts A, B, and C (2019).


Zuletzt aktualisiert 2019-04-06 um 13:02