Circulating factors cause proteinuria in parabiotic zebrafish

Müller-Deile J, Schenk H, Schroder P, Schulze K, Bolaños-Palmieri P, Siegerist F, Endlich N, Haller H, Schiffer M (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Kidney International Nature Publishing Group: Open Access Hybrid Model Option A

DOI: 10.1016/j.kint.2019.02.013

Abstract

Proteinuria can be induced by impairment of any component of the glomerular filtration barrier. To determine the role of circulating permeability factors on glomerular damage, we developed a parabiosis-based zebrafish model to generate a common circulation between zebrafish larvae. A morpholino-mediated knockdown of a podocyte specific gene (nephronectin) was induced in one zebrafish larva which was then fused to an un-manipulated fish. Notably, proteinuria and glomerular damage were present in the manipulated fish and in the parabiotically-fused partner. Thus, circulating permeability factors may be induced by proteinuria even when an induced podocyte gene dysregulation is the initiating cause.

Authors with CRIS profile

Mario Schiffer Lehrstuhl für Innere Medizin IV

Involved external institutions

Medizinische Hochschule Hannover (MHH) / Hannover Medical School DE Germany (DE) Universität Greifswald DE Germany (DE) Mount Desert Island Biological Laboratory US United States (USA) (US)

How to cite

APA:

Müller-Deile, J., Schenk, H., Schroder, P., Schulze, K., Bolaños-Palmieri, P., Siegerist, F.,... Schiffer, M. (2019). Circulating factors cause proteinuria in parabiotic zebrafish. Kidney International. https://dx.doi.org/10.1016/j.kint.2019.02.013

MLA:

Müller-Deile, J., et al. "Circulating factors cause proteinuria in parabiotic zebrafish." Kidney International (2019).

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