Proteomics of diphtheria toxoid vaccines reveals multiple proteins that are immunogenic and may contribute to protection of humans against Corynebacterium diphtheriae

Journal article


Publication Details

Author(s): Möller J, Kraner M, Sonnewald U, Sangal V, Tittlbach H, Winkler J, Winkler T, Melnikov V, Lang R, Sing A, Mattos-Guaraldi AL, Burkovski A
Journal: Vaccine
Publication year: 2019
ISSN: 0264-410X


Abstract

Introduced for mass immunization in the 1920s, vaccines against diphtheria are among the oldest and safest vaccines known. The basic principle of their production is the inactivation of purified diphtheria toxin by formaldehyde cross-linking, which converts the potentially fatal toxin in a completely harmless protein aggregate, which is still immunogenic. Since in addition to diphtheria toxin also other proteins may be secreted by Corynebacterium diphtheriae during cultivation, we assumed that diphtheria toxoid might not be the only component present in the vaccine. To address this question, we established a protocol to reverse formaldehyde cross-linking and carried out mass spectrometric analyses. Different secreted, membrane-associated and cytoplasmic proteins of C. diphtheriae were detected in several vaccine preparations from across the world. Based on these results, bioinformatics and Western blot analyses were applied to characterize if these proteins are immunogenic and may therefore support protection against C. diphtheriae. In frame of this study, we could show that the C. diphtheriae toxoid vaccines induce antibodies against different C. diphtheriae proteins and against diphtheria toxin secreted by Corynebacterium ulcerans, an emerging pathogen which is outnumbering C. diphtheriae as cause of diphtheria-like illness in Western Europe.


FAU Authors / FAU Editors

Burkovski, Andreas Prof. Dr.
Professur für Mikrobiologie
Kraner, Max Dr.
Lehrstuhl für Biochemie
Lang, Roland Prof. Dr.
Professur für Angeborene Immunität und Pathogenerkennung
Möller, Jens
Professur für Mikrobiologie
Sonnewald, Uwe Prof. Dr.
Lehrstuhl für Biochemie
Winkler, Thomas Prof. Dr.
Professur für Genetik
Winkler, Jürgen Prof. Dr.
Molekular-Neurologische Abteilung in der Neurologischen Klinik


External institutions with authors

Gabrichevsky Institute of Epidemiology and Microbiology
Northumbria University
Rio de Janeiro State University / Universidade do Estado do Rio de Janeiro (UERJ)


How to cite

APA:
Möller, J., Kraner, M., Sonnewald, U., Sangal, V., Tittlbach, H., Winkler, J.,... Burkovski, A. (2019). Proteomics of diphtheria toxoid vaccines reveals multiple proteins that are immunogenic and may contribute to protection of humans against Corynebacterium diphtheriae. Vaccine. https://dx.doi.org/10.1016/j.vaccine.2019.04.059

MLA:
Möller, Jens, et al. "Proteomics of diphtheria toxoid vaccines reveals multiple proteins that are immunogenic and may contribute to protection of humans against Corynebacterium diphtheriae." Vaccine (2019).

BibTeX: 

Last updated on 2019-23-05 at 16:53