Laboratory diagnosis of congenital CMV infection in newborns: Impact of pre-analytic factors
Kohmer N, Nagel AM, Berger A, Enders M, Hamprecht K, Korn K, Kortenbusch M, Überla K, Rabenau HF (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 115
Pages Range: 32-36
DOI: 10.1016/j.jcv.2019.03.017
Abstract
Background: To identify infants with congenital cytomegalovirus (cCMV) saliva polymerase chain reaction (PCR) is an ideal screening method. However, there are only few data on the influence of pre-analytic factors on the analytical sensitivity of the CMV PCR. Objectives: This study aimed to evaluate the performance of different swabbing materials, transport time and initial virus concentration regarding to the efficacy of recovery of CMV-DNA. Study design: Two CMV suspensions containing a high or low concentration of the laboratory strain AD 169 were prepared as test samples. Sampling was simulated by immersion of different swabs in these CMV suspensions and storing the swabs dry or in specified transport media. Transport conditions were modeled by storing the samples for defined time periods prior to DNA extraction and quantitative PCR analyses. Parallel analyses in two different laboratories allowed determination of lab to lab consistency. Results: The duration of storage under the conditions analysed did not have a major effect on the recovery efficiency for the swabbing materials tested. With exception of flocked dry swabs, all tested swabbing materials demonstrated good recovery of CMV DNA. The flocked swab/eNAT system showed the best overall performance. Conclusions: All tested swabbing materials (with exception of the flocked dry swabs) seem to be well suited for recovery of CMV DNA and appropriate for use for the diagnosis of cCMV infection in symptomatic cases and in general cCMV screening programs of newborns.
Authors with CRIS profile
Armin Michael Nagel
Professur für metabolische und funktionelle MR-Bildgebung
Klaus Überla
Institute of Clinical and Molecular Virology
Involved external institutions
Universitätsklinikum Frankfurt am Main (KGU)
Germany (DE)
Labor Prof. Gisela Enders MVZ GbR
Germany (DE)
Universitätsklinikum Tübingen
Germany (DE)
Germany (DE)
Labor Prof. Gisela Enders MVZ GbR
Germany (DE)
Universitätsklinikum Tübingen
Germany (DE)
How to cite
APA:
Kohmer, N., Nagel, A.M., Berger, A., Enders, M., Hamprecht, K., Korn, K.,... Rabenau, H.F. (2019). Laboratory diagnosis of congenital CMV infection in newborns: Impact of pre-analytic factors. Journal of Clinical Virology, 115, 32-36. https://dx.doi.org/10.1016/j.jcv.2019.03.017
MLA:
Kohmer, N., et al. "Laboratory diagnosis of congenital CMV infection in newborns: Impact of pre-analytic factors." Journal of Clinical Virology 115 (2019): 32-36.
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