Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane

Taylor RW, Mahmoodabadi RG, Rauschenberger V, Gießl A, Schambony A, Sandoghdar V (2019)

Publication Type: Journal article

Publication year: 2019

Journal

DOI: 10.1038/s41566-019-0414-6

Abstract

Many of the biological functions of a cell are dictated by the intricate motion of proteins within its membrane over a spatial range of nanometres to tens of micrometres and time intervals of microseconds to minutes. This rich parameter space is not accessible by fluorescence microscopy, but it is within reach of interferometric scattering (iSCAT) particle tracking. However, as iSCAT is sensitive even to single unlabelled proteins, it is often accompanied by a large speckle-like background, which poses a substantial challenge for its application to cellular imaging. Here, we employ a new image processing approach to overcome this difficulty and demonstrate tracking of transmembrane epidermal growth factor receptors with nanometre precision in all three dimensions at up to microsecond speeds and for durations of tens of minutes. We provide examples of nanoscale motion and confinement in ubiquitous processes such as diffusion in the plasma membrane, transport on filopodia and rotational motion during endocytosis.

Authors with CRIS profile

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light Germany (DE)

How to cite

APA:

Taylor, R.W., Mahmoodabadi, R.G., Rauschenberger, V., Gießl, A., Schambony, A., & Sandoghdar, V. (2019). Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane. Nature Photonics. https://dx.doi.org/10.1038/s41566-019-0414-6

MLA:

Taylor, Richard W., et al. "Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane." Nature Photonics (2019).

BibTeX: Download