Frontline Science: Aggregated neutrophil extracellular traps prevent inflammation on the neutrophil-rich ocular surface

Journal article


Publication Details

Author(s): Mahajan A, Grüneboom A, Petru L, Podolska M, Kling L, Maueröder C, Dahms F, Christiansen S, Günter L, Krenn V, Jünemann A, Bock F, Schauer C, Schett G, Hohberger B, Herrmann M, Munoz LE
Journal: Journal of Leukocyte Biology
Publication year: 2019
ISSN: 0741-5400


Abstract

Eye rheum is a physiological discharge, which accumulates at the medial angle of the healthy eye soon after opening in the morning. Microscopic evaluation of eye rheum revealed the presence of viable neutrophils, bacteria, epithelial cells, and particles, aggregated by neutrophil extracellular traps. We observed that in the evening, during eye closure, high C5a recruited neutrophils to the tear film and activated them. In this hypoxic area rich in CO
2
, neutrophils fight microbial aggressors by degranulation. Immediately after eye opening, the microenvironment of the ocular surface changes, the milieu gets normoxic, and loss of CO
2
induces subtle alkalinization of tear film. These conditions favored the formation of neutrophil extracellular traps (NETs) that initially covers the ocular surface and tend to aggregate by eyelid blinking. These aggregated neutrophil extracellular traps (aggNETs) are known as eye rheum and contain several viable neutrophils, epithelial cells, dust particles, and crystals packed together by NETs. Similar to aggNETs induced by monosodium urate crystals, the eye rheum shows a robust proteolytic activity that degraded inflammatory mediators before clinically overt inflammation occur. Finally, the eye rheum passively floats with the tear flow to the medial angle of the eye for disposal. We conclude that the aggNETs-based eye rheum promotes cleaning of the ocular surface and ameliorates the inflammation on the neutrophil-rich ocular surfaces.


FAU Authors / FAU Editors

Herrmann, Martin Prof. Dr.
Medizinische Klinik 3 - Rheumatologie und Immunologie
Hohberger, Bettina Dr.
Institut für Anatomie
Mahajan, Aparna
Medizinische Klinik 3 - Rheumatologie und Immunologie
Maueröder, Christian
Medizinische Klinik 3 - Rheumatologie und Immunologie
Podolska, Malgorzata
Medizinische Klinik 3 - Rheumatologie und Immunologie
Schett, Georg Prof. Dr. med.
Lehrstuhl für Innere Medizin III


External institutions with authors

Helmholtz-Zentrum Berlin für Materialien und Energie (HZB)
Kerckhoff-Klinik
Universität Köln
Universitätsmedizin Rostock


How to cite

APA:
Mahajan, A., Grüneboom, A., Petru, L., Podolska, M., Kling, L., Maueröder, C.,... Munoz, L.E. (2019). Frontline Science: Aggregated neutrophil extracellular traps prevent inflammation on the neutrophil-rich ocular surface. Journal of Leukocyte Biology. https://dx.doi.org/10.1002/JLB.HI0718-249RR

MLA:
Mahajan, Aparna, et al. "Frontline Science: Aggregated neutrophil extracellular traps prevent inflammation on the neutrophil-rich ocular surface." Journal of Leukocyte Biology (2019).

BibTeX: 

Last updated on 2019-23-05 at 16:38