Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging

Journal article

Publication Details

Author(s): Dilipkumar A, Al-Shemmary A, Kreiß L, Cvecek K, Carlé BE, Knieling F, Menezes JG, Thoma OM, Schmidt M, Neurath M, Waldner M, Friedrich O, Schürmann S
Journal: Advanced Science
Publication year: 2019
Volume: 6
Journal issue: 8
ISSN: 2198-3844


Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label-free in vivo imaging in small animals via side-viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label-free endomicroscopy for longitudinal studies for the first time.

FAU Authors / FAU Editors

Al-Shemmary, Alaa Dr.
Lehrstuhl für Medizinische Biotechnologie
Carlé, Birgitta-Elisabeth
Lehrstuhl für Medizinische Biotechnologie
Cvecek, Kristian Dr.
Lehrstuhl für Photonische Technologien
Dilipkumar, Ashwathama
Lehrstuhl für Medizinische Biotechnologie
Friedrich, Oliver Prof. Dr. Dr.
Lehrstuhl für Medizinische Biotechnologie
Knieling, Ferdinand Dr. med.
Kinder- und Jugendklinik
Kreiß, Lucas
Lehrstuhl für Medizinische Biotechnologie
Neurath, Markus Prof. Dr.
Lehrstuhl für Innere Medizin I
Schmidt, Michael Prof. Dr.-Ing.
Lehrstuhl für Photonische Technologien
Schürmann, Sebastian Dr. rer. nat.
Lehrstuhl für Medizinische Biotechnologie
Waldner, Maximilian Prof. Dr.
Professur für Funktionelle Bildgebung in der Medizin

How to cite

Dilipkumar, A., Al-Shemmary, A., Kreiß, L., Cvecek, K., Carlé, B.-E., Knieling, F.,... Schürmann, S. (2019). Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging. Advanced Science, 6(8).

Dilipkumar, Ashwathama, et al. "Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging." Advanced Science 6.8 (2019).


Last updated on 2019-04-06 at 13:08