Breast Cancer Cells Adapt Contractile Forces to Overcome Steric Hindrance

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autorinnen und Autoren: Condor M, Mark C, Gerum R, Grummel N, Bauer A, Garcia-Aznar JM, Fabry B
Zeitschrift: Biophysical Journal
Jahr der Veröffentlichung: 2019
Band: 116
Heftnummer: 7
Seitenbereich: 1305-1312
ISSN: 0006-3495


Abstract

Cell migration through the extracellular matrix is governed by the interplay between cell-generated propulsion forces, adhesion forces, and resisting forces arising from the steric hindrance of the matrix. Steric hindrance in turn depends on matrix porosity, matrix deformability, cell size, and cell deformability. In this study, we investigate how cells respond to changes in steric hindrance that arise from altered cell mechanical properties. Specifically, we measure traction forces, cell morphology, and invasiveness of MDA-MB 231 breast cancer cells in three-dimensional collagen gels. To modulate cell mechanical properties, we either decrease nuclear deformability by twofold overexpression of the nuclear protein lamin A or we introduce into the cells stiff polystyrene beads with a diameter larger than the average matrix pore size. Despite this increase of steric hindrance, we find that cell invasion is only marginally inhibited, as measured by the fraction of motile cells and the mean invasion depth. To compensate for increased steric hindrance, cells employ two alternative strategies. Cells with higher nuclear stiffness increase their force polarity, whereas cells with large beads increase their net contractility. Under both conditions, the collagen matrix surrounding the cells stiffens dramatically and carries increased strain energy, suggesting that increased force polarity and increased net contractility are functionally equivalent strategies for overcoming an increased steric hindrance.


FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Fabry, Ben Prof. Dr.
Lehrstuhl für Biophysik
Lehrstuhl für Biophysik
Gerum, Richard Dr.
Grummel, Nadine
Sonderforschungsbereich/Transregio 225: Von den Grundlagen der Biofabrikation zu funktionalen Gewebemodellen
Mark, Christoph Dr.
Lehrstuhl für Biophysik


Einrichtungen weiterer Autorinnen und Autoren

Universidad de Zaragoza


Zitierweisen

APA:
Condor, M., Mark, C., Gerum, R., Grummel, N., Bauer, A., Garcia-Aznar, J.M., & Fabry, B. (2019). Breast Cancer Cells Adapt Contractile Forces to Overcome Steric Hindrance. Biophysical Journal, 116(7), 1305-1312. https://dx.doi.org/10.1016/j.bpj.2019.02.029

MLA:
Condor, Mar, et al. "Breast Cancer Cells Adapt Contractile Forces to Overcome Steric Hindrance." Biophysical Journal 116.7 (2019): 1305-1312.

BibTeX: 

Zuletzt aktualisiert 2019-18-04 um 12:38