A Multiple Piccolino-RIBEYE Interaction Supports Plate-Shaped Synaptic Ribbons in Retinal Neurons

Journal article


Publication Details

Author(s): Müller T, Gierke K, Joachimsthaler A, Sticht H, Izsvak Z, Hamra FK, Fejtová A, Ackermann F, Garner CC, Kremers J, Brandstätter JH, Regus-Leidig H
Journal: Journal of Neuroscience
Publication year: 2019
Volume: 39
Journal issue: 14
Pages range: 2606-2619
ISSN: 0270-6474


Abstract

Active zones at chemical synapses are highly specialized sites for the regulated release of neurotransmitters. Despite a high degree of active zone protein conservation in vertebrates, every type of chemical synapse expresses a given set of protein isoforms and splice variants adapted to the demands on neurotransmitter release. So far, we know little about how specific active zone proteins contribute to the structural and functional diversity of active zones. In this study, we explored the nanodomain organization of ribbon-type active zones by addressing the significance of Piccolino, the ribbon synapse-specific splice variant of Piccolo, for shaping the ribbon structure. We followed up on previous results, which indicated that rod photoreceptor synaptic ribbons lose their structural integrity in a knockdown of Piccolino. Here, we demonstrate an interaction between Piccolino and the major ribbon component RIBEYE that supports plate-shaped synaptic ribbons in retinal neurons. In a detailed ultrastructural analysis of three different types of retinal ribbon synapses in Piccolo/Piccolino-deficient male and female rats, we show that the absence of Piccolino destabilizes the superstructure of plate-shaped synaptic ribbons, although with variable manifestation in the cell types examined. Our analysis illustrates how the expression of a specific active zone protein splice variant (e.g., Piccolino) contributes to structural diversity of vertebrate active zones.


FAU Authors / FAU Editors

Brandstätter, Johann Helmut Prof. Dr.
Lehrstuhl für Tierphysiologie
Fejtová, Anna Prof. Dr.
Professur für Molekulare Psychiatrie
Gierke, Kaspar
Lehrstuhl für Tierphysiologie
Joachimsthaler, Anneka
Augenklinik
Kremers, Jan Prof. Dr.
Professur für Experimentelle Augenheilkunde
Müller, Tanja Dr.
Lehrstuhl für Tierphysiologie
Regus-Leidig, Hanna PD Dr.
Lehrstuhl für Tierphysiologie
Sticht, Heinrich Prof. Dr.
Professur für Bioinformatik


External institutions with authors

Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Helmholtz-Gemeinschaft
University of Texas Southwestern Medical Center (UT Southwestern)


How to cite

APA:
Müller, T., Gierke, K., Joachimsthaler, A., Sticht, H., Izsvak, Z., Hamra, F.K.,... Regus-Leidig, H. (2019). A Multiple Piccolino-RIBEYE Interaction Supports Plate-Shaped Synaptic Ribbons in Retinal Neurons. Journal of Neuroscience, 39(14), 2606-2619. https://dx.doi.org/10.1523/JNEUROSCI.2038-18.2019

MLA:
Müller, Tanja, et al. "A Multiple Piccolino-RIBEYE Interaction Supports Plate-Shaped Synaptic Ribbons in Retinal Neurons." Journal of Neuroscience 39.14 (2019): 2606-2619.

BibTeX: 

Last updated on 2019-16-04 at 13:23