Characterization of mast cell-derived rRNA-containing microvesicles and their inflammatory impact on endothelial cells

Journal article


Publication Details

Author(s): Elsemueller AK, Tomalla V, Gaertner U, Troidl K, Jeratsch S, Graumann J, Baal N, Hackstein H, Lasch M, Deindl E, Preissner KT, Fischer S
Journal: The FASEB Journal
Publication year: 2019
Volume: 33
Journal issue: 4
Pages range: 5457-5467
ISSN: 0892-6638
eISSN: 1530-6860


Abstract

Tissue-resident mast cells (MCs) are well known for their role in inflammatory responses and allergic and anaphylactic reactions, but they also contribute to processes of arterial remodeling. Although ribosomes and cytosolic RNAs are located around secretory granules in mature MCs, their functional role in MC responses remains unexplored. Previous studies by our group characterized extracellular RNA (eRNA) as an inflammatory and pathogenetic factor in vitro and in vivo. In the present study, RNA-containing MCs and eRNA were located in close proximity to growing collateral arteries in vivo. In vitro, various agonists were found to induce the degranulation of MCs and the concomitant release of eRNA in association with microvesicles (MVs). The liberation of eRNA from MCs was abolished by MC stabilizers or by preventing the increase of intracellular Ca2+ in MCs. eRNA was found to be mainly contained inside MVs, as demonstrated by electron microscopy and immunocytochemistry. The exposure to and the uptake of MC-released MVs by cultured endothelial cells increased their expression of cytokines, such as monocyte chemoattractant protein or IL-6, in a dose- and time-dependent manner. These results indicate that RNA-containing MC-derived MVs are likely to be involved in inflammatory responses, relevant, for example, to processes of vascular remodeling.


FAU Authors / FAU Editors

Hackstein, Holger Prof. Dr. med.
Professur für Transfusionsmedizin und Cell-Engineering


External institutions with authors

Justus-Liebig-Universität Gießen
Ludwig-Maximilians-Universität (LMU)
Max Planck Institute for Heart and Lung Research / Max-Planck-Institut für Herz- und Lungenforschung


How to cite

APA:
Elsemueller, A.-K., Tomalla, V., Gaertner, U., Troidl, K., Jeratsch, S., Graumann, J.,... Fischer, S. (2019). Characterization of mast cell-derived rRNA-containing microvesicles and their inflammatory impact on endothelial cells. The FASEB Journal, 33(4), 5457-5467. https://dx.doi.org/10.1096/fj.201801853RR

MLA:
Elsemueller, Ann-Kathrin, et al. "Characterization of mast cell-derived rRNA-containing microvesicles and their inflammatory impact on endothelial cells." The FASEB Journal 33.4 (2019): 5457-5467.

BibTeX: 

Last updated on 2019-10-09 at 09:33