Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6

Beitrag in einer Fachzeitschrift


Details zur Publikation

Autor(en): Caballero GG, Schmidt S, Schnoelzer M, Schlötzer-Schrehardt U, Knospe C, Ludwig AK, Manning JC, Muschler P, Kaltner H, Kopitz J, Gabius HJ
Zeitschrift: Cell and Tissue Research
Jahr der Veröffentlichung: 2019
Band: 375
Heftnummer: 3
Seitenbereich: 665-683
ISSN: 0302-766X


Abstract

Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their binding partners and the profile of localization. Having identified the chicken galectin-related interfiber protein (C-GRIFIN) as lens-specific protein present in the main body of adult lens, we here report its interaction with lens proteins in ligand blotting. The assumption for pairing with -, - and -crystallins was ascertained by mass spectrometric detection of their presence in eluted fractions obtained by affinity chromatography. Biochemical and immunohistochemical monitoring revealed protein presence from about 3-day-old embryos onwards, mostly in the cytoplasm of elongated posterior cells, later in secondary lens fiber cells. On the level of gene expression, its promoter was activated by transcription factor L-Maf alone and together with Pax6 like a crystallin gene, substantiating C-GRIFIN's status as lens-specific galectin. Using this combined strategy for counterreceptor and expression profiling by bio- and histochemical methods including light, electron and fluorescence microscopy, respective monitoring in lens development can now be taken to the level of the complete galectin family.


FAU-Autoren / FAU-Herausgeber

Schlötzer-Schrehardt, Ursula apl. Prof. Dr.
Medizinische Fakultät


Zusätzliche Organisationseinheit(en)
Augenklinik


Autor(en) der externen Einrichtung(en)
Deutsches Krebsforschungszentrum (DKFZ)
Ludwig-Maximilians-Universität (LMU)
Promega GmbH
Ruprecht-Karls-Universität Heidelberg


Zitierweisen

APA:
Caballero, G.G., Schmidt, S., Schnoelzer, M., Schlötzer-Schrehardt, U., Knospe, C., Ludwig, A.-K.,... Gabius, H.-J. (2019). Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6. Cell and Tissue Research, 375(3), 665-683. https://dx.doi.org/10.1007/s00441-018-2931-x

MLA:
Caballero, Gabriel Garcia, et al. "Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6." Cell and Tissue Research 375.3 (2019): 665-683.

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Zuletzt aktualisiert 2019-22-03 um 06:56