Loss of the novel Vcp (valosin containing protein) interactor Washc4 interferes with autophagy-mediated proteostasis in striated muscle and leads to myopathy in vivo
Kustermann M, Manta L, Paone C, Kustermann J, Lausser L, Wiesner C, Eichinger L, Clemen CS, Schröder R, Kestler HA, Sandri M, Rottbauer W, Just S (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 14
Pages Range: 1911-1927
Journal Issue: 11
DOI: 10.1080/15548627.2018.1491491
Abstract
VCP/p97 (valosin containing protein) is a key regulator of cellular proteostasis. It orchestrates protein turnover and quality control in vivo, processes fundamental for proper cell function. In humans, mutations in VCP lead to severe myo- and neuro-degenerative disorders such as inclusion body myopathy with Paget disease of the bone and frontotemporal dementia (IBMPFD), amyotrophic lateral sclerosis (ALS) or and hereditary spastic paraplegia (HSP). We analyzed here the in vivo role of Vcp and its novel interactor Washc4/Swip (WASH complex subunit 4) in the vertebrate model zebrafish (Danio rerio). We found that targeted inactivation of either Vcp or Washc4, led to progressive impairment of cardiac and skeletal muscle function, structure and cytoarchitecture without interfering with the differentiation of both organ systems. Notably, loss of Vcp resulted in compromised protein degradation via the proteasome and the macroautophagy/autophagy machinery, whereas Washc4 deficiency did not affect the function of the ubiquitin-proteasome system (UPS) but caused ER stress and interfered with autophagy function in vivo. In summary, our findings provide novel insights into the in vivo functions of Vcp and its novel interactor Washc4 and their particular and distinct roles during proteostasis in striated muscle cells.
Authors with CRIS profile
Involved external institutions
Universität Ulm
Germany (DE)
Universität zu Köln
Germany (DE)
University of Padua / Universita degli Studi di Padova
Italy (IT)
Germany (DE)
Universität zu Köln
Germany (DE)
University of Padua / Universita degli Studi di Padova
Italy (IT)
How to cite
APA:
Kustermann, M., Manta, L., Paone, C., Kustermann, J., Lausser, L., Wiesner, C.,... Just, S. (2018). Loss of the novel Vcp (valosin containing protein) interactor Washc4 interferes with autophagy-mediated proteostasis in striated muscle and leads to myopathy in vivo. Autophagy, 14(11), 1911-1927. https://dx.doi.org/10.1080/15548627.2018.1491491
MLA:
Kustermann, Monika, et al. "Loss of the novel Vcp (valosin containing protein) interactor Washc4 interferes with autophagy-mediated proteostasis in striated muscle and leads to myopathy in vivo." Autophagy 14.11 (2018): 1911-1927.
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